RADIOIMMUNOASSAY FOR BRAIN NATRIURETIC PEPTIDE (BNP) DETECTION OF BNP IN CANINE BRAIN
RADIOIMMUNOASSAY FOR BRAIN NATRIURETIC PEPTIDE (BNP) DETECTION OF BNP IN CANINE BRAIN
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DOI：
10.1016/s0006-291x(89)80186-x
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发表时间：
1989-01-16
影响因子：
3.1
通讯作者：
IMURA, H
中科院分区：
文献类型：
--
作者：
ITOH, H;NAKAO, K;IMURA, H
我们建立了一种高度敏感和特异性的BNP放射免疫测定法（RIA）。我们的RIA在猪和犬的大脑中检测到bnp样免疫反应性（-LI），但在人、猴和大鼠的大脑中未检测到BNP-LI。BNP-LI在猪和犬的大脑中均有广泛分布，在延髓中浓度最高。同时测定的ANP-LI浓度最高的是中脑和嗅球。高效凝胶渗透色谱联用RIA发现，BNP- li的主要成分被洗脱在小分子量（3K）的合成BNP的位置。这些结果表明，BNP的RIA可以作为研究BNP生理作用的有用工具。
We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.