Testing the usefulness of hydrogen and compound-specific stable isotope analyses in seabird feathers: a case study in two sympatric Antarctic storm-petrels
Testing the usefulness of hydrogen and compound-specific stable isotope analyses in seabird feathers: a case study in two sympatric Antarctic storm-petrels
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DOI：
10.1007/s00227-017-3224-8
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发表时间：
2017-09
期刊：
影响因子：
2.4
通讯作者：
P. Quillfeldt;S. Thorn;Benjamin Richter;M. Nabte;N. Coria;J. Masello;M. Massaro;Verónica C Neves
中科院分区：
文献类型：
--
作者：
P. Quillfeldt;S. Thorn;Benjamin Richter;M. Nabte;N. Coria;J. Masello;M. Massaro;Verónica C Neves
氮和碳稳定同位素为研究海鸟的生态隔离、食物选择和空间分布提供了工具。然而，稳定同位素的解释经常受到缺乏同位素基线数据的阻碍。在这项研究中，提出了两种技术，以克服这些不足进行了测试：化合物特定的同位素分析的氨基酸（AA-CSIA）和氢稳定同位素比（HSIA）的分析。比较了两只同域风暴海燕的羽毛。这两个物种，黑腹风暴海燕热带海燕和威尔逊风暴海燕大洋海燕，在海洋沃茨中换羽，饮食组成不同。对于HSIA，还调查了一系列具有广泛饮食和非繁殖分布的物种。碳同位素值的差异表明在空间分布的差异，因此，在同位素基线值，换羽。成羽的散装氮分析没有发现物种的营养级差异。然而，AA-CSIA检测到明显的差异，符合预期的营养水平：黑腹风暴海燕喂养的营养水平高于威尔逊的风暴海燕。氢值也不同的物种之间，但与预期相反，高度丰富的黑腹风暴海燕，但少得多的丰富威尔逊的风暴海燕。七种海燕的氢数据挑战的建议，耗尽δD值表示较高比例的等渗性鱼类。目前的研究结果表明，氢比的差异可能是由于这些海燕在不同的海洋区域蜕皮。氨基酸特定的稳定同位素分析是有用的估计同位素基线，从而真正的营养水平，而氢同位素没有。
Nitrogen and carbon stable isotopes provide tools to investigate ecological segregation, prey choice and spatial distribution in seabirds. However, the interpretation of stable isotopes is frequently hampered by a lack of isotopic baseline data. In this study, two techniques proposed to overcome such shortages were tested: compound-specific isotope analyses of amino acids (AA-CSIA) and the analysis of hydrogen stable isotope ratios (HSIA). Feathers of two sympatric storm-petrels were compared. The two species, Black-bellied storm-petrelsFregetta tropicaand Wilson’s storm-petrelsOceanites oceanicus, moult in oceanic waters and differ in diet composition. For HSIA, a range of species with broad diet and non-breeding distribution was also investigated. Differences in carbon isotope values suggested differences in the spatial distribution and thus, in isotopic baseline values, during moult. Bulk nitrogen analyses of adult feathers did not detect species differences in trophic level. However, AA-CSIA detected clear differences in trophic levels in line with expectations: Black-bellied storm-petrels fed at a higher trophic level than Wilson‘s storm-petrels. Hydrogen values also differed between the species, but contrary to expectations were highly enriched in Black-bellied storm-petrels, but much less enriched in Wilson’s storm-petrels. Hydrogen data of seven petrel species challenge the suggestion that depleted δD values indicate a higher percentage of isosmotic fish. The present results suggest that the difference in hydrogen ratios may be explained by these petrels moulting in different ocean zones. Amino acid-specific stable isotope analyses were useful for estimating isotopic baselines and thus true trophic levels, whereas hydrogen isotopes were not.