Water and salt intake induced by hypothalamic implants of cholinergic and adrenergic agents.
Water and salt intake induced by hypothalamic implants of cholinergic and adrenergic agents.
复制标题
下丘脑植入胆碱能和肾上腺素能药物诱导水和盐的摄入。
DOI：
10.1152/ajplegacy.1969.216.6.1418
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
S. Taleisnik
中科院分区：
文献类型：
--
作者：
E. Chiaraviglio;S. Taleisnik
METHODSSubjects。选用体重200 ~ 280 g的雄性白化大鼠。他们被单独关在笼子里，可以自由获取食物和水。Inz $蚂蚁。所有动物均植入双壁插管。套管由两根针组成；外罩直径0.6毫米，内罩直径0.3毫米，与内罩紧密贴合。内针可以拧入外针，两根套管的尖端达到同一水平。在乙醚麻醉下，利用立体定向仪在颅骨上钻一个孔，将双壁插管植入第三脑室。在de Groot寰椎7.0-7.4 AP坐标(5)的中线位置植入套管，并降低至+ 0.5-1.5的水平。用牙水泥将它们固定在颅骨上，并缝合皮肤。
METHODSSubjects. Male albino rats weighing 200-280 g were used. They were caged individually and had free access to food and water.Inz $ ants. Double-walled cannulas were implanted in all animals. The cannulas consisted of two needles; the outer one was 0.6 mm in diameter and the inner was 0.3 mm and fitted tightly inside. The inner needle could screw into the outer one, the tips of both cannulas reaching the same level. The double-walled cannula was implanted, under ether anesthesia, into the third ventricle through a hole drilled in the skull by means of a stereotaxic instrument. The cannulas were implanted in the midline at the level of 7.0-7.4 AP coordinates of de Groot’s atlas (5) and lowered to the level of+ 0.5-1.5. They were fixed to the skull with dental cement and the skin was sutured.