Mapping the mosaic of ancestral genotypes in a cultivar of oilseed rape (Brassica napus) selected via pedigree breeding
Mapping the mosaic of ancestral genotypes in a cultivar of oilseed rape (Brassica napus) selected via pedigree breeding
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DOI：
10.1139/g03-031
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发表时间：
2003-06-01
期刊：
影响因子：
3.1
通讯作者：
Lydiate, DJ
中科院分区：
文献类型：
--
作者：
Sharpe, AG;Lydiate, DJ
最近的油菜育种已经产生了低芥子油苷品种，可产生适合动物饲料的蛋白质粉。低芥子油苷的特性被引入现代油菜品种“Bronowski”中，该品种在大多数其他方面农艺性较差。现代品种中“Bronowski”基因型的残留片段可能会导致产量下降、抗寒性较差和含油量较低。现代油菜品种甘蓝型油菜“Tapidor”中“Bronowski”基因型的数量和分布是使用源自“Tapidor”与其高芥子油苷祖先杂交的分离群体进行研究的。使用 65 个信息丰富的芸苔属 RFLP 探针对该群体进行了分析，并构建了基于 77 个多态性位点的分离的遗传连锁图。该图谱确定了“Tapidor”中供体基因型的 15 个残留片段，它们总共占据了欧洲油菜基因组的大约 29%。绘制控制分离群体中总种子芥子油苷积累变异的基因座已确定了三个基因座，它们共同解释了该性状> 90%的变异。所有这些基因座都位于“Tapidor”基因组的供体片段中。这一结果表明，传统育种计划难以从优良材料中消除供体基因型的残留片段。
Recent oilseed rape breeding has produced low glucosinolate cultivars that yield proteinaceous meal suitable for animal feed. The low glucosinolate character was introduced into modern cultivars from Brassica napus 'Bronowski', a cultivar that is agronomically inferior in most other respects. Residual segments of 'Bronowski' genotype in modern cultivars probably cause reduced yield, poorer winter hardiness, and lower oil content. The quantity and distribution of the 'Bronowski' genotype in the modern oilseed rape cultivar Brassica napus 'Tapidor' was investigated using a segregating population derived from a cross between 'Tapidor' and its high glucosinolate progenitor. This population was analyzed with 65 informative Brassica RFLP probes and a genetic linkage map, based on the segregation at 77 polymorphic loci, was constructed. The mapping identified 15 residual segments of donor genotype in 'Tapidor', which together occupy approximately 29% of the B. napus genome. Mapping the loci that control variation for the accumulation of total seed glucosinolates in the segregating population has identified three loci that together explain >90% of the variation for this character. All of these loci are in donor segments of the 'Tapidor' genome. This result shows the extent to which conventional breeding programmes have difficulty in eliminating residual segments of donor genotype from elite material.