Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York
Genetic Differences Between Culex pipiens f. molestus and Culex pipiens pipiens (Diptera: Culicidae) in New York
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f. 淡色库蚊之间的遗传差异
DOI：
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发表时间：
2007
影响因子：
2.1
通讯作者：
D. Norris
中科院分区：
文献类型：
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作者：
Rebekah J. Kent;L. Harrington;D. Norris
摘要淡色库蚊自体型蚊虫的定义和系统发育位置几十年来一直困扰着昆虫学家。我们鉴定了Cx和Cx之间的遗传差异。淡色假单胞菌P.piiens(L.)和CX.在前面描述的SH60片段中发现了一种名为F.molestus Forsk aka L的同源序列。该片段的单链构象多态性分析、克隆和测序表明，该片段在Cx个体内和个体间具有高度的多态性。P.piiens，与来自遥远地区的个体共享共同的SH60变种。相比之下，CX。来自纽约市的每一个人都含有一个SH60变异体，这在任何其他CX中都没有被发现。对淡色假单胞菌标本进行了分析。支持微卫星分析显示，Cx之间的基因流动显著但减少。Popiens和Cx.在纽约与Cx.纽约和加利福尼亚州的淡色库蚊种群。在关于CX起源的两种截然不同的假说的背景下讨论了结果。淡色库蚊种群。
Abstract The definition and phylogenetic placement of the autogenous molestus form of Culex pipiens has puzzled entomologists for decades. We identified genetic differences between Cx. p. pipiens (L.) and Cx. pipiens f. molestus Forskål in the SH60 fragment described previously. Single-strand conformation polymorphism analysis, cloning, and sequencing of this fragment demonstrated high polymorphism within and among individual Cx. p. pipiens, with common SH60 variants shared among individuals from distant locations. In contrast, Cx. pipiens f. molestus from New York City each contained a single SH60 variant, which was not identified in any other Cx. p. pipiens specimens analyzed. Supporting microsatellite analysis demonstrated significant but reduced gene flow between Cx. p. pipiens and Cx. pipiens f. molestus in New York relative to Cx. p. pipiens populations in New York and California. Results are discussed in the context of two contrasting hypotheses regarding the origin of Cx. pipiens f. molestus populations.
DOI：
10.4269/ajtmh.2005.73.336
发表时间：
2005-08-01
影响因子：
3.3
作者：
Kent, RJ;Norris, DE
通讯作者：
Norris, DE