Strategies in fed-batch cultivation on the production performance of Lactobacillus salivarius I 24 viable cells
Strategies in fed-batch cultivation on the production performance of Lactobacillus salivarius I 24 viable cells
复制标题
DOI：
10.1007/s10068-016-0217-1
复制
发表时间：
2016-10-01
影响因子：
2.9
通讯作者：
Bin Ariff, Arbakariya
中科院分区：
文献类型：
--
作者：
Ming, Lim Chi;Halim, Murni;Bin Ariff, Arbakariya
利用2-L搅拌槽生物反应器，研究了补料分批培养在提高唾液乳杆菌I24菌体产量方面的潜在应用。将3种不同的恒定投喂速率(0.1%、0.05%和0.033%L/h)应用于FBC5中，评价其对碳代谢的影响。与分批培养模式相比，在补料分批培养模式中观察到了随着乳酸合成的减少而建立的细胞碳通量。在L/h进料速度为0.05x10(10)cfu/m L的条件下，恒定滤池获得的活细胞数是分批培养的8倍。这使得以CFBC消耗的葡萄糖为基础的活细胞产量比分批培养高26倍(11.3×10(12)cfu/g(葡萄糖))。本研究表明，CFBC工艺简单，过程控制设备用量少，具有提高益生菌生产的工业潜力。
The potential use of fed-batch cultivation (FBC) for improvement of the production of Lactobacillus salivarius I 24 biomass for subsequent use as probiotics was studied using a 2-L stirred-tank bioreactor. Three different constant feeding rates (0.1, 0.05, and 0.033 L/h) were applied in FBC5 and their effect on carbon metabolism was evaluated. The carbon flux for cell built-up with reduction in lactic acid synthesis was observed in the fed-batch as compared to the batch cultivation mode. The viable cell number obtained in the constant FBC (CFBC) operated at a feeding rate of 0.05 L/h was 8 times higher (10.7x10(10) CFU/mL) than that recorded in the batch cultivation. This gave the viable cell yield based on glucose consumed for CFBC of 26 times higher (11.3x10(12) CFU/g(Glucose)) than the batch cultivation. This study demonstrated CFBC, which is simple with minimal use of process control equipment, has an industrial potential for improvement of probiotic production.