Sperm DNA fragmentation in Chinese couples with unexplained recurrent pregnancy loss
Sperm DNA fragmentation in Chinese couples with unexplained recurrent pregnancy loss
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中国不明原因反复流产夫妇的精子 DNA 碎片
DOI：
10.4103/aja.aja_60_19
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发表时间：
2020-05-01
影响因子：
2.9
通讯作者：
Zhang, Ai-Jun
中科院分区：
文献类型：
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作者：
Zhu, Xiao-Bin;Chen, Qian;Zhang, Ai-Jun
我们旨在通过对2013年5月至2018年8月期间经历过反复妊娠丢失(RPL)的中国夫妇的回顾性观察研究，研究精子DNA片段化与中国人群中反复妊娠丢失(RPL)的关系。研究人群包括来自RPL夫妇的461名男性和来自对照组的411名男性(因女性原因而通过体外受精临床怀孕的夫妇)。使用精液样本进行常规精液分析、精子染色质分析和显微镜(高倍)形态分析。对精液样本进行了体积、精子数和活动度的评估。计算精子DNA断裂指数(DFI)，得到中位数DFI。男性分为正常(37.8%；DFI≤15.0%)、中度(33.6%；15.0%；DFI<；30.0%)、重度(28.6%；DFI≥30.0%)。RPL组男性DNA严重断裂的比例(42.3%)显著高于对照组(13.1%)，而DNA正常水平的男性比例(22.8%)显著低于对照组(54.7%)。随后的分析还表明，精子DNA碎片率与精子前进活动率(r=−0.47，P<；0.001)和活动精子总数(r=−0.31，P<；0.001)呈中等程度的负相关。我们发现RPL与精子DNA片段化呈正相关。结果表明，精子DNA损伤增加与RPL有关。
We aimed to study the association between sperm DNA fragmentation and recurrent pregnancy loss (RPL) in the Chinese population via a retrospective observational study of Chinese couples who had experienced RPL between May 2013 and August 2018. The study population included 461 men from couples with RPL and 411 men from a control group (couples with clinical pregnancy via in vitro fertilization owing to female causes). Routine semen analysis, sperm chromatin analysis, and microscopic (high-power) morphological analysis were performed using semen samples. Semen samples were assessed for volume, sperm count, and motility. The sperm DNA fragmentation index (DFI) was calculated, and the median DFI was obtained. Men were categorized as having normal (37.8%; DFI ≤ 15.0%), moderate (33.6%; 15.0% < DFI < 30.0%), or severe (28.6%; DFI ≥ 30.0%) DNA fragmentation levels. The percentage of men with severe DNA fragmentation was significantly higher in the RPL (42.3%) group than that in the control group (13.1%), whereas the percentage of men with normal levels of DNA fragmentation was significantly lower in the RPL group (22.8%) than that in the control group (54.7%). Subsequent analysis also demonstrated that the sperm DNA fragmentation rate had a moderate reverse correlation with the sperm progressive motility rate (r = −0.47, P < 0.001) and the total motile sperm count (r = −0.31, P < 0.001). We found a positive correlation between RPL and sperm DNA fragmentation. The results suggest that increased sperm DNA damage is associated with RPL.