A time and cost efficient approach to functional and structural assessment of living neuronal tissue
A time and cost efficient approach to functional and structural assessment of living neuronal tissue
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DOI：
10.1016/j.jneumeth.2013.01.016
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发表时间：
2013-03-30
影响因子：
3
通讯作者：
Sagdullaev, Botir T.
中科院分区：
文献类型：
--
作者：
Toychiev, Abduqodir H.;Sagdullaev, Bakhodir;Sagdullaev, Botir T.
在这篇手稿中，我们描述了一个协议，用于捕获活的神经元组织的生理和结构特性。这种方法的一个重要方面是它的灵活性和快速周转时间。这是一个简化的过程，包括记录电生理神经元活动，钙成像和结构分析。这是通过将完整的组织放置在改良的Millicell Biopore衬垫上来实现的。Biopore膜将组织悬浮在灌注溶液中，允许完全获得营养物质，氧气和药理学试剂。固定膜的环确保其结构稳定性;可使用镊子夹持环，而不接触滤器或组织，以便在多个设置之间轻松转移。我们表明，组织很容易粘附到膜的表面，其整个表面是可见的透射光和可访问的记录和成像，并保持响应生理刺激的时间延长。(C)2013爱思唯尔有限公司版权所有。
In this manuscript, we describe a protocol for capturing both physiological and structural properties of living neuronal tissue. An essential aspect of this method is its flexibility and fast turnaround time. It is a streamlined process that includes recording of electrophysiological neuronal activity, calcium imaging, and structural analysis. This is accomplished by placing intact tissue on a modified Millicell Biopore insert. The Biopore membrane suspends the tissue in the perfusion solution, allowing for complete access to nutrients, oxygen, and pharmacological agents. The ring that holds the membrane ensures its structural stability; forceps can be used to grip the ring without contacting the filter or the tissue, for easy transfer between multiple setups. We show that tissue readily adheres to the surface of the membrane, its entire surface is visible in transmitted light and accessible for recording and imaging, and remains responsive to physiological stimuli for extended periods of time. (C) 2013 Elsevier B.V. All rights reserved.