Expression of fragile X chromosome in human-rodent somatic cell hybrids.
Expression of fragile X chromosome in human-rodent somatic cell hybrids.
复制标题
脆弱 X 染色体在人类-啮齿动物体细胞杂交体中的表达。
DOI：
10.1007/bf01535636
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Davidson,RL
中科院分区：
文献类型：
--
作者：
Warren,ST;Davidson,RL
脆性X染色体，与一种常见的X连锁精神发育迟滞有关，在细胞学上最常观察到的是Xq 28带长臂远端附近的一个缺口或脆性位点。该位点的表达是可变的，并且依赖于降低的胸苷酸池。为了研究这种脆性位点在外来遗传背景下的行为，从来自脆性X连锁精神发育迟滞男性患者的仓鼠细胞和淋巴母细胞的杂交中分离出种间体细胞杂交体。分析了三个含有人X染色体的杂交细胞系。用5-氟脱氧尿苷诱导后，所有三个杂交种在约10%的中期分裂相中表达了脆性位点。我们的数据表明，在带Xq 27中的脆性位点的表达既不依赖于人类基因组的完整性，也不依赖于人类常染色体基因的表达。
The fragile X chromosome, associated with a common form of X-linked mental retardation, is cytologically observed most often as a gap or fragile site near the distal end of the long arm in band Xq28. Expression of this site is variable and dependent upon lowered thymidylate pools. In order to examine the behavior of this fragile site in a foreign genetic background, interspecific somatic cell hybrids were isolated from crosses of hamster cells and lymphoblastoid cells derived from male patients with fragile X-linked mental retardation. Three hybrid cell lines containing the human X chromosome were analyzed. Following induction with 5-fluorodeoxyuridine, all three hybrids expressed the fragile site in approximately 10% of the metaphases examined. Our data indicate that expression of the fragile site in band Xq27 is dependent neither on the integrity of the human genome nor on the expression of human autosomal genes.