Multimycotoxin Determination in Tunisian Farm Animal Feed
Multimycotoxin Determination in Tunisian Farm Animal Feed
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DOI：
10.1111/1750-3841.14948
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发表时间：
2019-11-24
影响因子：
3.9
通讯作者：
Berrada, Houda
中科院分区：
文献类型：
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作者：
Juan, Cristina;Oueslati, Souheib;Berrada, Houda
首次对在突尼斯销售的动物饲料中存在的真菌毒素进行了评估。采用QuEChERS方法对122份突尼斯市售的家禽（43份）、牛（35份）、兔（12份）、羊（16份）和马（16份）的饲料样品中22种真菌毒素（enniatins、beauvericins、赭曲霉毒素A、黄曲霉毒素、交替胺醇单甲醚、交替胺醇、tentoxin、玉米赤霉烯酮、脱氧雪腐镰刀菌醇、3-乙酰脱氧雪腐镰刀菌醇、15-乙酰脱氧雪腐镰刀菌醇、雪腐镰刀菌醇、新向日葵醇、二乙酰氧基旋甲醇、T-2毒素和HT-2毒素）的天然含量进行了分析。分析物的检测和定量采用液相色谱和气相色谱耦合串联质谱法。该方法线性良好（R = 0.996），灵敏度高，定量限为0.1 ng/g（三乙酰脱氧雪梨酚）~ 225 ng/g（3-乙酰脱氧雪梨酚）。85%的分析样本呈阳性。家禽（n = 43）和家兔（n = 12）的饲料样品污染最严重。最常见的真菌毒素是Enniatin B，在马饲料中含量为0.5 ng/g，在家禽饲料中含量为40 ng/g，其次是脱氧雪腐镰刀菌醇，在牛饲料中含量为16 ng/g，在家禽饲料中含量为250 ng/g。没有一个超过欧盟对动物饲料的建议限值。最多观察到五种不同的真菌毒素（26%）共发生，5%的样品中记录到多达八种真菌毒素。此外，不同镰刀菌毒素的出现率较高。即使没有明确显示毒理学问题，污染是一个真实的事实，可能会对肉类生产和食品安全产生影响。
Mycotoxins presence was evaluated in animal feed marketed in Tunisia for the first time ever. A QuEChERS method was performed to analyze the natural copresence of 22 mycotoxins (enniatins, beauvericin, ochratoxin A, aflatoxins, alternariol monomethyl ether, alternariol, tentoxin, zearalenone, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, nivalenol, neosolaniol, diacetoxyscirpenol, T-2 toxin, and HT-2 toxin) in 122 Tunisian marketed feed samples, intended for poultry (n = 43), cattle (n = 35), rabbit (n = 12), sheep (n = 16), and horse (n = 16). Analytes detection and quantification were done using both liquid chromatography and gas chromatography coupled to tandem mass spectrometry. The analytical method showed good linearity (R > 0.996) and sensitivity, the limits of quantification ranged from 0.1 ng/g (enniatin A1) to 225 ng/g (3-acetyldeoxynivalenol). Eighty-five percent of the analyzed samples were positive. Poultry (n = 43) and rabbit (n = 12) feed samples were the most contaminated. Enniatin B was the most prevalent mycotoxin with values ranged between 0.5 ng/g for horse feed and 40 ng/g for poultry feed, followed by deoxynivalenol detected from 16 ng/g in cattle feed to 250 ng/g in poultry feed. None exceeded the limits set by EU recommendations for animal feed. Mycotoxins co-occurrence was observed at most by five different mycotoxins (26%) and up to eight mycotoxins was recorded in 5% of samples. Furthermore, a relatively high copresence rate of different fusariotoxins was registered. Even if no toxicological concern was clearly revealed, the contamination is a real fact and will probably present influence on meat production and on food safety.