Cell-Free Mitochondrial DNA as a Potential Biomarker for Astronauts' Health.
Cell-Free Mitochondrial DNA as a Potential Biomarker for Astronauts' Health.
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DOI：
10.1161/jaha.121.022055
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发表时间：
2021-11-02
影响因子：
5.4
通讯作者：
Goukassian DA
中科院分区：
文献类型：
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作者：
Bisserier M;Shanmughapriya S;Rai AK;Gonzalez C;Brojakowska A;Garikipati VNS;Madesh M;Mills PJ;Walsh K;Arakelyan A;Kishore R;Hadri L;Goukassian DA
据报道，宇航员在国际空间站上执行短期和长期任务后，会出现与太空旅行相关的压力源，例如微重力或辐射暴露。尽管采取了风险缓解策略，但对健康的不利影响仍然令人担忧。因此，需要开发新的诊断工具以促进生理应激的早期检测。我们测量了 14 名宇航员在发射前 10 天、着陆当天和返回后 3 天血浆中循环游离线粒体 DNA 的水平。我们的结果显示，在着陆当天和返回后 3 天，血浆中的无细胞线粒体 DNA 显着增加，宇航员之间的变异性高达约 2 至 355 倍。此外，外周血单核细胞的基因表达分析显示炎症、氧化应激和 DNA 损伤标记物显着增加。我们的研究表明，无细胞线粒体 DNA 丰度可能是太空飞行期间与微重力、辐射和其他环境因素相关的压力或免疫反应的生物标志物。
Space travel–associated stressors such as microgravity or radiation exposure have been reported in astronauts after short‐ and long‐duration missions aboard the International Space Station. Despite risk mitigation strategies, adverse health effects remain a concern. Thus, there is a need to develop new diagnostic tools to facilitate early detection of physiological stress. We measured the levels of circulating cell‐free mitochondrial DNA in blood plasma of 14 astronauts 10 days before launch, the day of landing, and 3 days after return. Our results revealed a significant increase of cell‐free mitochondrial DNA in the plasma on the day of landing and 3 days after return with vast ~2 to 355‐fold interastronaut variability. In addition, gene expression analysis of peripheral blood mononuclear cells revealed a significant increase in markers of inflammation, oxidative stress, and DNA damage. Our study suggests that cell‐free mitochondrial DNA abundance might be a biomarker of stress or immune response related to microgravity, radiation, and other environmental factors during space flight.