Molecular phylogeography of endangered sharp-snouted pitviper (Deinagkistrodon acutus; Reptilia, Viperidae) in Mainland China
Molecular phylogeography of endangered sharp-snouted pitviper (Deinagkistrodon acutus; Reptilia, Viperidae) in Mainland China
复制标题
中国大陆濒危尖吻蝰蛇（Deinagkistrodon acutus；爬行纲、蝰科）的分子系统发育地理学
DOI：
10.1016/j.ympev.2007.05.019
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发表时间：
2007-09-01
影响因子：
4.1
通讯作者：
Zhao, Ermi
中科院分区：
文献类型：
--
作者：
Huang, Song;He, Shunping;Zhao, Ermi
采用系统发育和种群遗传学方法，研究了尖吻蝮蛇（Deinagkistrodon acutus）的种群地理结构。对86个日本血吸虫个体的线粒体ND2基因（NADH脱氢酶亚基2）进行了全序列测定。测定了我国14个分布区的尖吻蝮蛇属（acutus）。根据系统发育分析、诊断位点分布、单倍型网络分析和AMOVA系统分析结果，对整个D.可观察到尖吻鲈种群。在地理上，一条由湖泊、河流和山脉（鄱阳湖、赣江到武夷山南端）组成的线，导致了隔离，并大致垂直地将范围分为两部分，整个人口分为两个主要谱系（西部和东部）。分叉树显示出总体上由西向东的扩散趋势。数据符合隔离距离（IBD）模型。单倍型网络中的星形簇、Fs统计量的显著负值和单峰错配分布都表明四个地区最近的人口扩张。结果表明，孤立、扩散、瓶颈和扩张共同构成了D. acutus在单倍型网络中，中心单倍型占绝对优势，中频率单倍型较少，衍生单倍型中单倍型占绝对优势（73.1%），大多数单倍型与中心单倍型仅通过一步突变相连，错配分布的单峰钟状曲线不对称，峰向左移动，这些都表明整个D.尖吻鲈种群是一个遗传多样性较低的年轻种群。根据这些数据，应优先考虑黄山单元的保护行动。(c)2007年爱思唯尔公司All rights reserved.
Using phylogenetic and population genetic approaches, the present study reports the phylogeographic structure of the sharp-snouted pitviper (Deinagkistrodon acutus), a threatened snake species with commercial and medicinal importance in China. The entire mitochondrial ND2 gene (NADH dehydrogenase subunit 2) sequences of 86 individuals of D. acutus from 14 localities across its range in China were determined. Based on the results of phylogenetic analyses, distribution of diagnostic sites, haplotype network, and AMOVA hierarchical analysis, an cast-west division of the whole D. acutus population could be observed. Geographically, a line formed by a lake, river, and mountain chain (the Poyang Lake, Gan River to the southern end of the Wuyi Mountains), results in vicariance and approximately vertically splits the range into two and the whole population into two main lineages (western and eastern). The bifurcating tree suggested generally west to east dispersal trend. The data fit the isolation by distance (IBD) model well. Star-like clusters in haplotype network, significantly negative values of Fs statistics, and unimodal mismatch distributions all suggest recent demographic expansions in four areas. The results show that isolation, dispersal, bottleneck, and expansion jointly constitute the history of D. acutus. In a haplotype network, the excessive predominance of central haplotypes, few medium-frequency haplotypes, predominance (73.1 %) of the singletons among the derived haplotypes, most of which are connected to the central haplotype by only one mutational step, unsymmetrical campanulate unimodal curve of mismatch distributions and leftwards shift of the peaks, all suggest that the whole D. acutus population is a young population with low genetic diversity. Based on the data, the first priority for conservation action should be given to the Huangshan unit. (c) 2007 Elsevier Inc. All rights reserved.