Differential effects of long and short carbon nanotubes on the gas-exchange region of the mouse lung
Differential effects of long and short carbon nanotubes on the gas-exchange region of the mouse lung
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DOI：
10.3109/17435390.2011.626533
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发表时间：
2012-12-01
期刊：
影响因子：
5
通讯作者：
Donaldson, Ken
中科院分区：
文献类型：
--
作者：
Muehlfeld, Christian;Poland, Craig A.;Donaldson, Ken
我们假设暴露于短或长多壁碳纳米管（MWCNT）后，肺实质的炎症反应和形态学特征不同。小鼠通过咽部滴入单剂量的载体，短或长MWCNT。分析24 h支气管肺泡灌洗液（BALF）的炎症反应，并用体视学分析肺组织的形态学改变。短MWCNT具有更强的诱导多形核细胞的潜力，而长MWCNT增加了BALF中白细胞介素-6的水平。肺泡间隔纤维化仅在短时间MWCNT中观察到。II型肺细胞肥大仅在长MWCNT中检测到。肺泡表面积没有减少，也没有II型细胞增生的迹象。我们观察到，根据所应用的MWCNT的大小，在肺实质中对短和长MWCNT有轻微的炎症和病理反应。
We hypothesise that inflammatory response and morphological characteristics of lung parenchyma differ after exposure to short or long multi-walled carbon nanotubes (MWCNT). Mice were subjected to a single dose of vehicle, short or long MWCNT by pharyngeal aspiration. Bronchoalveolar lavage fluid (BALF) obtained at 24 h was analysed for inflammatory reaction and lung tissue was analysed for morphological alterations using stereology. Short MWCNT had stronger potential to induce polymorphonuclear cells whereas long MWCNT increased interleukin-6 levels in BALF. Alveolar septal fibrosis was only observed with short MWCNT. Type II pneumocyte hypertrophy was only detected with long MWCNT. There was no reduction in total alveolar surface area and no sign of type II cell hyperplasia. We observed mild inflammatory and pathological responses to short and long MWCNT in the lung parenchyma depending on the size of the applied MWCNT.