Baculovirus as a highly efficient expression vector in insect and mammalian cells.
Baculovirus as a highly efficient expression vector in insect and mammalian cells.
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杆状病毒是昆虫和哺乳动物细胞中高效的表达载体。
DOI：
10.1111/j.1745-7254.2005.00078.x
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发表时间：
2005-04
影响因子：
8.2
通讯作者：
Hu YC
中科院分区：
文献类型：
--
作者：
Hu YC
杆状病毒已被广泛用于在昆虫细胞中生产重组蛋白。自从发现杆状病毒可以有效地转导哺乳动物细胞以来，杆状病毒的应用得到了极大的扩展。对杆状病毒介导的基因在昆虫和哺乳动物细胞中表达的前景和不足进行了综述。讨论了近年来在基因调控、病毒载体制备、体内和体外基因治疗研究、疫苗载体制备等方面的应用进展，并着重指出了克服存在的瓶颈的努力。
Baculovirus has been widely used for the production of recombinant proteins in insect cells. Since the finding that baculovirus can efficiently transduce mammalian cells, the applications of baculovirus have been greatly expanded. The prospects and drawbacks of baculovirus‐mediated gene expression, either in insect or in mammalian cells, are reviewed. Recent progresses in expanding the applications to studies of gene regulation, viral vector preparation, in vivo and ex vivo gene therapy studies, generation of vaccine vectors, etc are discussed and the efforts directed towards overcoming the existing bottlenecks are particularly emphasized.
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影响因子：
4.2
作者：
Barsoum, J;Brown, R;Boyce, FM
通讯作者：
Boyce, FM
影响因子：
1.8
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Clay, WC;Condreay, JP;Lorenz, JJ
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Lorenz, JJ
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4.9
作者：
Delaney, WE;Miller, TG;Isom, HC
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Isom, HC
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13.5
作者：
Delaney, WE;Isom, HC
通讯作者：
Isom, HC
影响因子：
5.1
作者：
Beck, NB;Sidhu, JS;Omiecinski, CJ
通讯作者：
Omiecinski, CJ