An untargeted metabolomics approach to investigate the wine-processed mechanism of Scutellariae radix in acute lung injury
An untargeted metabolomics approach to investigate the wine-processed mechanism of Scutellariae radix in acute lung injury
复制标题
DOI：
10.1016/j.jep.2020.112665
复制
发表时间：
2020-05-10
影响因子：
5.4
通讯作者：
Yang, Ming
中科院分区：
文献类型：
--
作者：
Hu, Lianqi;Wang, Yaqi;Yang, Ming
民族药理学相关性：黄芪（SR）是最受欢迎的传统中药之一。粗SR（CSR）和葡萄酒加工SR（WSR）是两种最常见的商业规格。根据中医理论，酒的加工增加了SR作用的倾向性和方向性，从而增强了其清上焦肺湿热的功效。WSR治疗肺部疾病的药理机制相关研究有限，需要扩大。研究目的：本报告的目的是通过评估脂多糖（LPS）诱导的急性肺损伤（ALI）模型中CSR和WSR之间血浆代谢产物的变化来确定相关的生物学途径，从而揭示葡萄酒加工在SR中的潜在机制。材料和方法：用CSR和WSR治疗LPS诱导的大鼠ALI。通过测定炎性细胞因子含量和组织病理学检查，探讨中药复方对炎症反应的影响。接下来，通过超高效液相色谱-四极杆飞行时间质谱法（UPLC-QTOF-MS）对大鼠血浆样品进行代谢分析。然后，使用主成分分析（PCA）来提供所有组的概述，并使用正交偏最小二乘判别分析（OPLS-DA）来最大化区分并呈现所有组之间代谢物的差异。与CSR相比，WSR通过降低炎性因子，包括一氧化氮（NO），肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）。在UPLC-QTOF-MS技术的基础上，非靶向代谢组学揭示了疗效的不均匀性。在LPS诱导的大鼠血浆中发现了16种生物标志物。通路分析表明，CSR通过调节异常的鞘脂代谢途径对ALI起作用;然而，WSR介导的ALI治疗主要与逆转视黄醇代谢途径和色氨酸代谢途径的异常有关。结论：本报告从血浆代谢产物的角度研究了SR的潜在葡萄酒加工机制。此外，这项工作提供了一个新的和有价值的见解，解释中药加工机制的整体方式。
Ethnopharmacological relevance: Scutellariae radix (SR) is one of the most popular traditional Chinese medicines (TCM). Crude SR (CSR) and wine-processed SR (WSR) are the two most common commercial specifications. According to the theories of TCM, wine-processing increases the inclination and direction of SR's actions, thereby strengthening its efficacy in clearing the upper-energizer lung damp heat. The pharmacological mechanism-related research on WSR for the treatment of lung disease is limited and needs to be expanded.Aim of the study: The aim of this report was to identify the relevant biological pathways by assessing changes in plasma metabolites between CSR and WSR in a lipopolysaccharide (LPS)-induced acute lung injury (ALI) model, and thus, revealed the potential mechanism of wine processing in SR.Materials and methods: Rats with LPS-induced ALI were treated with CSR and WSR. The contents of inflammatory cytokines and histopathological examination were determined to explore the effects of CSR and WSR. Next, the metabolic profiling of rat plasma samples was performed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Then, principal component analysis (PCA) were used to provide an overview for all of the groups and orthogonal partial least squares-discriminant analysis (OPLS-DA) was utilized to maximize the discrimination and present the differences in the metabolite between all of the groups.Results: WSR exhibited a more remarkable effect on improving ALI than CSR by reducing the levels of inflammatory factors, including nitric oxide (NO), tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-8 (IL-8). On the basis of UPLC-QTOF-MS technology, an unequal curative effect was revealed by nontargeting metabolomics. Sixteen biomarkers were discovered in the plasma of LPS-induced rats. Pathway analysis indicated that CSR acted on ALI by regulating the abnormal sphingolipid metabolism pathways; however, an WSR-mediated cure of ALI was linked primarily to reversing the abnormality of retinol metabolism pathways and tryptophan metabolism pathways.Conclusions: This report examined the underlying wine-processing mechanism of SR from the perspective of plasma metabolites. In addition, this work provided a novel and valuable insight into interpretation of the processing mechanisms of TCM in a holistic way.