Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Apiotrichum porosum DSM27194
Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Apiotrichum porosum DSM27194
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DOI：
10.1016/j.fuel.2020.119811
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发表时间：
2021-02-04
期刊：
影响因子：
7.4
通讯作者：
Jiang, Min
中科院分区：
文献类型：
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作者：
Qian, Xiujuan;Zhou, Xinhai;Jiang, Min
从废弃有机物中提取的挥发性脂肪酸（VFA）为燃料和油脂化学品的生产提供了经济的原料。在这项研究中，VFA转化为微生物脂质的产油酵母Apiotrichum porosum DSM 27194进行了研究。A.多孔菌DSM 27194能够同化含有不同比例的乙酸、丙酸和丁酸的不同VFA混合物用于脂质生产。以葡萄糖为初始碳源，VFA混合物为碳源和缓冲剂，采用两阶段自动补料分批发酵策略，在VFA比例为6：1：1（乙酸：丙酸：丁酸）时，获得干细胞质量浓度为26.5g/L，脂肪含量为36.2%的发酵液。脂肪酸组成分析表明，来自VFA的脂质更适合于生物柴油的生产相比，葡萄糖作为碳源。此外，含有VFA的玉米秸秆水解液作为原料。令人兴奋的是，A。多孔菌DSM 27194能快速消耗水解液中的各种碳源，同时产生28.0g/L的干细胞质量，含44.3%的脂质。本研究为A. porosum DSM 27194和两阶段自动补料分批发酵的经济发酵策略，用于从低价值VFA衍生的原料生产微生物脂质。
Volatile fatty acids (VFA) derived from waste organics provide economical feedstocks for fuels and oleochemicals production. In this study, the valorization of VFA into microbial lipids by the oleaginous yeast Apiotrichum porosum DSM27194 was investigated. A. porosum DSM 27194 was able to assimilate different VFA mixtures containing acetic, propionic and butyric acid at different ratios for lipid production. By using a two-stage automated fed-batch fermentation strategy with glucose as initial carbon source and VFA mixtures as both carbon source and buffering agent, a dry cell mass concentration of 26.5 g/L with lipid content of 36.2% was achieved with the VFA ratio of 6:1:1 (acetic acid:propionic acid:butyric acid). Fatty acid composition profiles indicated that the lipid derived from VFA is better suited for biodiesel production compared to glucose as carbon source. Moreover, corn stover hydrolysate containing VFA was applied as raw feedstock. Excitingly, A. porosum DSM 27194 could consume all kinds of carbon source within the hydrolysate fast and simultaneously resulting in 28.0 g/L of dry cell mass containing 44.3% of lipid. This study provides a promising oleaginous yeast chassis of A. porosum DSM 27194 and an economical fermentation strategy of two-stage automated fed-batch fermentation for microbial lipid production from low-value VFA derived feedstocks.