Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice
Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice
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DOI：
10.1016/s1534-5807(01)00025-9
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发表时间：
2001-08-01
影响因子：
11.8
通讯作者：
Richter, JD
中科院分区：
文献类型：
--
作者：
Tay, J;Richter, JD
CPEB 是一种序列特异性 RNA 结合蛋白，在脊椎动物卵母细胞成熟过程中调节翻译。成年雌性 CPEB 基因敲除小鼠的卵巢退化，缺乏卵母细胞；来自妊娠中期胚胎的卵巢含有在粗线期被捕获的卵母细胞。雄性 CPEB 缺失小鼠也含有在粗线期停滞的生殖细胞。基因敲除小鼠的生殖细胞含有片段化染色质，表明同源染色体粘附或突触可能存在缺陷。两种含有 CPE 的联会复合蛋白 mRNA 在体外和体内与 CPEB 相互作用，含有缩短的聚 (A) 尾，并且在无效小鼠中大多无法与多聚核糖体沉淀。在这些动物中没有检测到联会复合体。因此，CPEB 通过调节联会复合体的形成来控制生殖细胞分化。
CPEB is a sequence-specific RNA binding protein that regulates translation during vertebrate oocyte maturation. Adult female CPEB knockout mice contained vestigial ovaries that were devoid of oocytes; ovaries from mid-gestation embryos contained oocytes that were arrested at the pachytene stage. Male CPEB null mice also contained germ cells arrested at pachytene. The germ cells from the knockout mice harbored fragmented chromatin, suggesting a possible defect in homologous chromosome adhesion or synapsis. Two CPE-containing synaptonemal complex protein mRNAs, which interact with CPEB in vitro and in vivo, contained shortened poly(A) tails and mostly failed to sediment with polysomes in the null mice. Synaptonemal complexes were not detected in these animals. CPEB therefore controls germ cell differentiation by regulating the formation of the synaptonemal complex.