Dehydroepiandrosterone reduces proliferation and differentiation of 3T3-L1 preadipocytes.
Dehydroepiandrosterone reduces proliferation and differentiation of 3T3-L1 preadipocytes.
复制标题
Dehydroepiandrosterone 可减少 3T3-L1 前脂肪细胞的增殖和分化。
DOI：
10.1006/bbrc.1998.8996
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发表时间：
1998
影响因子：
3.1
通讯作者：
McIntosh,MK
中科院分区：
文献类型：
--
作者：
Lea-Currie,YR;Wen,P;McIntosh,MK
The purpose of these studies was to determine whether the antiobesity actions of dehydroepiandrosterone (DHEA) and DHEA-sulfate (DHEAS) observedin vivoare due to an influence on proliferation and/or differentiation in monolayer cultures of 3T3-L1 preadipocytes. For the proliferation study (Exp. 1), cells were grown in plating medium containing DHEA at 0, 5, 25, 50, or 100 μM for 1–4 d. DHEAS was added at the 100 μM level only. For the differentiation study (Exp. 2), cultures were grown in plating medium containing DHEA at 0, 5, 30, 60, 120, or 240 μM for 2–6 d. DHEAS was added at the 240 μM level only. In Exp. 3, the effect of DHEA on mature adipocytes was determined by exposing adipocytes grown in plating medium to DHEA at 0, 75, 125, and 250 μM for 1–4 d. In Exp. 1, preadipocyte proliferation decreased as the level of DHEA increased in cultures of 3T3-L1 cells. DHEAS had no effect on preadipocyte proliferation. The antiproliferative effect of DHEA was partially reversed by the addition of 1 μM mevalonic acid to proliferating cultures containing 25 μM DHEA. In Exp. 2, preadipocyte differentiation decreased as the level of DHEA in the cultures increased. In contrast, neither DHEAS nor mevalonic acid treatment influenced preadipocyte differentiation. In Exp. 3, markers of preadipocyte differentiation decreased as the level and duration of DHEA treatment increased in cultures of mature adipocytes. These data support the hypothesis that DHEA, but not DHEAS, is the active form of the steroid that attenuates obesity via altering preadipocyte proliferation and differentiation. The addition of 1 μM mevalonic acid to cultures of 3T3-L1 preadipocytes partially reversed DHEA's antiproliferative effects.
