Characterization of antennal chemosensilla and associated odorant binding as well as chemosensory proteins in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae).
Characterization of antennal chemosensilla and associated odorant binding as well as chemosensory proteins in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae).
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寄生蜂 Microplitis mediator（膜翅目：茧蜂科）触角化学感受器和相关气味结合以及化学感受蛋白的表征
DOI：
10.1038/s41598-018-25996-3
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发表时间：
2018-05-16
影响因子：
4.6
通讯作者：
Zhang YJ
中科院分区：
文献类型：
--
作者：
Wang SN;Shan S;Liu JT;Li RJ;Lu ZY;Dhiloo KH;Khashaveh A;Zhang YJ
气味结合蛋白（OBP）和化学感受蛋白（CSP）在昆虫触角化学感受器中的表达被认为是重要的化学感受。本研究对中红侧沟茧蜂触角感器的形态特征进行了详细描述，并对触角内OBP和CSP的表达模式进行了分析。In M.介体，触角上观察到8种类型的感器。锥形感器2型和锥形感器S.具有壁孔的板状幼体可能参与嗅觉感知，而s.具有顶端气孔的basiconica 1型和basiconica 3型可能具有味觉功能。在M的18个OBP和3个CSP中，mediator中，10个OBP和2个CSP仅在触角中表达或主要在触角中表达。原位杂交结果表明，这12个触角富集的OBP和CSP分别对应于5种形态的触角感受器，包括S。basiconica（1-3型），s. placodea和s.腔锥在触角内，OBP和CSP基因大多只在一种类型的感器中表达，表明它们在检测特殊类型的化学分子方面的作用是不同的。本研究结果为进一步研究OBP和CSP在寄生蜂触角中的生理功能奠定了基础。
Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) expressed in antennal chemosensilla are believed to be important in insect chemoreception. In the current study, we fully described the morphological characteristics of the antennal sensilla in parasitoid wasp Microplitis mediator and analyzed the expression patterns of OBPs and CSPs within the antennae. In M. mediator, eight types of sensilla were observed on the antennae. Sensilla basiconica type 2 and s. placodea with wall pores may be involved in olfactory perception, whereas s. basiconica type 1 and type 3 with tip pores may play gustatory functions. Among the 18 OBPs and 3 CSPs in M. mediator, 10 OBPs and 2 CSPs were exclusively or primarily expressed in the antennae. In situ hybridization experiments indicated that the 12 antennae-enriched OBPs and CSPs were mapped to five morphological classes of antennal sensilla, including s. basiconica (type 1–3), s. placodea and s. coeloconica. Within the antennae, most of OBP and CSP genes were expressed only in one type of sensilla indicating their differentiated roles in detection of special type of chemical molecules. Our data will lay a foundation to further study the physiological roles of OBPs and CSPs in antennae of parasitoid wasps.
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DOI：
10.1046/j.1432-1327.1999.00438.x
发表时间：
1999-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Angeli, S;Ceron, F;Pelosi, P
通讯作者：
Pelosi, P
影响因子：
2.5
作者：
Bleeker, MAK;Smid, HM;Vet, LEM
通讯作者：
Vet, LEM
影响因子：
4.2
作者：
Li, Jiancheng;Coudron, Thomas A.;Zhang, Qingwen
通讯作者：
Zhang, Qingwen
影响因子：
4.2
作者：
Colazza, S;Salerno, G;Wajnberg, E
通讯作者：
Wajnberg, E
影响因子：
4
作者：
Pelosi P;Iovinella I;Felicioli A;Dani FR
通讯作者：
Dani FR