Human T cell activation. II. A new activation pathway used by a major T cell population via a disulfide-bonded dimer of a 44 kilodalton polypeptide (9.3 antigen).
Human T cell activation. II. A new activation pathway used by a major T cell population via a disulfide-bonded dimer of a 44 kilodalton polypeptide (9.3 antigen).
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DOI：
10.1084/jem.161.6.1513
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发表时间：
1985-06-01
期刊：
影响因子：
--
通讯作者：
Hansen JA
中科院分区：
文献类型：
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作者：
Hara T;Fu SM;Hansen JA
在之前的研究 (17-21) 中，单克隆抗体 (mAb) 9.3 已被证明可与大部分人类 T 细胞发生反应，其中包括 T4+ 辅助/诱导 T 细胞和 T8+ 细胞毒性 T 细胞。在这项研究中，mAb 9.3 被证明可以沉淀 44 kD 多肽的二硫键二聚体。共调制实验表明该分子未与 T3/Ti 或 T11 抗原连接。 mAb 9.3 在 12-o-十四烷酰佛波醇-13-乙酸酯 (TPA) 存在的情况下能够诱导 T 细胞增殖。这种效应不依赖于单核细胞。 mAb 9.3 和 TPA 激活的 T 细胞与白细胞介素 2 (IL-2) 受体表达和 IL-2 分泌的增加相关。即使添加了 IL-1 或 IL-2，mAb 9.3 也不会激活 T 细胞。在 TPA 存在的情况下，T3 复合物的调节不会消除 mAb 9.3 诱导的 T 细胞增殖。这些结果表明 9.3 抗原可能充当仅限于 T 细胞亚群的激活途径的受体。
In previous studies (17-21), monoclonal antibody (mAb) 9.3 has been shown to react with a major population of human T cells, which include T4+ helper/inducer T cells and T8+ cytotoxic T cells. In this investigation, mAb 9.3 was shown to precipitate a disulfide-bonded dimer of a 44 kD polypeptide. Comodulation experiments showed that this molecule is not linked to T3/Ti or T11 antigens. mAb 9.3 was capable of inducing T cell proliferation in the presence of 12-o-tetradecanoyl phorbol-13-acetate (TPA). This effect was monocyte-independent. T cell activation with mAb 9.3 and TPA was associated with increases in interleukin 2(IL-2) receptor expression and IL-2 secretion. mAb 9.3 did not activate T cells, even with the addition of IL-1 or IL-2. Modulation of the T3 complex did not abolish mAb 9.3-induced T cell proliferation in the presence of TPA. These results suggest that the 9.3 antigen may serve as a receptor for an activation pathway restricted to a T cell subset.