Inhibition of azoreductase activity by antibodies against cytochromes P-450 and P-448.
Inhibition of azoreductase activity by antibodies against cytochromes P-450 and P-448.
复制标题
细胞色素 P-450 和 P-448 抗体抑制偶氮还原酶活性。
DOI：
10.1016/0006-291x(81)91546-1
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发表时间：
1981
影响因子：
3.1
通讯作者：
Peisach,J
中科院分区：
文献类型：
--
作者：
Fujita,S;Okada,Y;Peisach,J
用苯巴比妥(PB)和3-甲基胆蒽(3-MC)诱导小鼠肝微粒体偶氮还原酶活性。抗细胞色素P-450的抗体抑制PB处理的动物的偶氮还原酶活性，而抗细胞色素P-448的抗体抑制3-MC处理的动物的肝脏偶氮还原酶活性，每种抗体抑制约90%。这些抗体对微粒体7-乙氧基香豆素-O-脱乙基酶活性也有相同程度的抑制作用。结果表明，肝微体固氮还原酶活性几乎完全依赖于细胞色素P-450和P-448，其他微体成分的贡献可以忽略不计。
Hepatic microsomal azoreductase activity in mice was induced with phenobarbital (PB) and 3-methylcholanthrene (3-MC). Antibodies against cytochrome P-450 inhibited azoreductase activity of PB-treated animals while antibodies against cytochrome P-448 inhibited liver azoreductase activity of 3-MC-treated animals, each by about 90%. These antibodies also inhibited microsomal 7-ethoxycoumarin-O-deethylase activity to the same extent. It is concluded that hepatic microsomal azoreductase activity is almost totally dependent on cytochromes P-450 and P-448 and the contribution, if any, of other microsomal components is negligible.