Identification of unique lipoprotein subclasses for visceral obesity by component analysis of cholesterol profile in high-performance liquid chromatography
Identification of unique lipoprotein subclasses for visceral obesity by component analysis of cholesterol profile in high-performance liquid chromatography
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DOI：
10.1161/01.atv.0000155017.60171.88
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发表时间：
2005-03-01
影响因子：
8.7
通讯作者：
Yamashita, S
中科院分区：
文献类型：
--
作者：
Okazaki, M;Usui, S;Yamashita, S
目的 - 内脏脂肪积累对冠心病发展的贡献先前已有报道，但内脏脂肪积累与血清脂蛋白亚类之间的关系尚不清楚。方法和结果 - 我们检查了 62 名患有内脏脂肪综合征或肥胖的男性受试者（年龄 22 至 67 岁）的脂蛋白亚类与内脏脂肪积累的关系。通过计算机辅助高效液相色谱法测定极低密度、低密度和高密度脂蛋白亚类（VLDL、LDL 和 HDL）中的胆固醇水平。通过计算机断层扫描测量皮下脂肪面积和内脏脂肪面积。皮下脂肪面积与所有脂蛋白亚类的胆固醇水平之间没有显着相关性。相反，内脏脂肪面积与 VLDL 和 LDL 亚类（大 LDL 除外）呈正相关（P < 0.002），但与大、中 HDL 亚类呈负相关（P < 0.001）。分别调整血清胆固醇、甘油三酯、HDL胆固醇和LDL胆固醇后，观察到的小和极小LDL亚类的正相关性仍然显着（P < 0.005），但在调整LDL胆固醇后，大LDL亚类的显着负相关性（P < 0.005）。结论 - 这些发现表明，这种简单的高效液相色谱方法可用于轻松检测和评估脂蛋白亚类的异常分布。
Objective - The contribution of visceral fat accumulation to the development of coronary heart disease was previously reported, but the relation between visceral fat accumulation and serum lipoprotein subclasses was unknown.Methods and Results - We examined the relation of lipoprotein subclasses with visceral fat accumulation in 62 male subjects ( aged 22 to 67 years) with visceral fat syndrome or obesity. Cholesterol levels in very low - density, low-density, and high-density lipoprotein subclasses (VLDL, LDL, and HDL) were determined by computer-assisted high-performance liquid chromatography. Subcutaneous fat area and visceral fat area were measured by computed tomographic scanning. There was no significant correlation between the subcutaneous fat area and the cholesterol levels in all lipoprotein subclasses. In contrast, the visceral fat area was correlated positively ( P < 0.002) with VLDL and LDL subclasses, except for large LDL, but negatively ( P < 0.001) with those in large and medium HDL subclasses. The observed positive correlations of small and very small LDL subclasses remained significant ( P < 0.005) after adjustment for serum cholesterol, triglycerides, HDL cholesterol, and LDL cholesterol, respectively, but a significant negative correlation ( P < 0.005) of large LDL was obtained after adjustment for LDL cholesterol.Conclusion - These findings indicate that this simple high-performance liquid chromatography method may be applied for easy detection and evaluation of abnormal distribution of lipoprotein subclasses.