Screening for congenital adrenal hyperplasia: the Delfia Screening Test overestimates serum 17-hydroxyprogesterone in preterm infants.
Screening for congenital adrenal hyperplasia: the Delfia Screening Test overestimates serum 17-hydroxyprogesterone in preterm infants.
复制标题
先天性肾上腺增生的筛查：Delfia 筛查测试高估了早产儿的血清 17-羟基孕酮。
DOI：
10.1542/peds.97.1.100
复制
发表时间：
1996
期刊：
影响因子：
8
通讯作者：
C. Cronin
中科院分区：
文献类型：
--
作者：
S. al Saedi;H. Dean;W. Dent;E. Stockl;C. Cronin
目的
比较通过定量血清放射免疫测定法（RIA）（包括提取步骤）和筛查荧光免疫测定法（FIA）对早产儿血斑测定的17-羟孕酮（17-OHP）水平。
方法
受试者为39名胎龄小于31周的健康婴儿。每名婴儿每周都有血液采样，并对每个样本进行RIA和FIA。
结果
在28至41周的受孕后年龄的227个样本。通过RIA测量的平均+/-SD 17-OHP为11.4 +/-11.1 nmol/L（0.4 +/-0.4 μ g/dL），并随时间推移而降低。通过FIA测量的平均+/-SD 17-OHP为38.96 +/-37.3 nmol/L，大于17-OHP（RIA）。Log（delta FIA-RIA）与孕后年龄呈负相关（R2 = 0.39）。
结论
FIA血斑筛查高估了早产儿17-OHP水平，不应用于确定该人群先天性肾上腺皮质增生的可能性。我们已经放弃了FIA筛查体重小于1500 g的婴儿先天性肾上腺皮质增生症。
OBJECTIVE
To compare 17-hydroxyprogesterone (17-OHP) levels measured by quantitative serum radioimmunoassay (RIA), including an extraction step, and by screening fluoroimmunoassay (FIA) on blood spots in preterm infants.
METHODS
Subjects were 39 healthy infants born at less than 31 weeks' gestational age. Each infant had weekly blood sampling, and RIA and FIA were performed on each sample.
RESULTS
Two hundred twenty-seven samples were taken at 28 to 41 weeks' postconceptional age. Mean +/- SD 17-OHP measured by RIA was 11.4 +/- 11.1 nmol/L (0.4 +/- 0.4 micrograms/dL), and decreased over time. Mean +/- SD 17-OHP measured by FIA was 38.96 +/- 37.3 nmol/L, greater than 17-OHP (RIA). Log(delta FIA-RIA) was inversely related to postconceptional age (R2 = .39).
CONCLUSION
Screening FIA of blood spots overestimates levels of 17-OHP in preterm infants and should not be used to determine the likelihood of congenital adrenal hyperplasia in this population. We have abandoned FIA screening for congenital adrenal hyperplasia in infants weighing less than 1500 g.