[Standardization and issues of rheumatoid factor measurement].
[Standardization and issues of rheumatoid factor measurement].
复制标题
类风湿因子测定的标准化及问题[J].
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
H. Yoshida
中科院分区：
文献类型：
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作者：
Y. Imafuku;H. Yoshida
目标
揭示日本类风湿因子（RF）测定的现状。
方法
2003年，在改进RF测量的委员会成员中进行了一项小规模调查，使用了基于WHO RF参考和混合血清进行估值的制造商RF参考材料。还进行了全国范围的问卷调查。2004年12月，向日本流变学大学认证的356所教育机构发送了调查问卷。
结果
小规模调查显示，测量的RF值的实验室间差异，特别是使用RF参考，并在合并血清较少。在问卷调查（187份回复，回收率，53%）中，定性或半定量RF测量方法在少数机构中使用，定量方法在大量机构中使用，主要采用乳胶免疫测定法（浊度法或比浊法）。测量仪器多种多样。参考区间的上限在5 IU/ml ~ 40 IU/ml范围内广泛分布，表明RF测量存在明显的实验室间差异。许多研究机构使用制造商推荐的参考区间。
结论
射频测量存在显著的实验室间差异。显然，主要问题之一是缺乏参考材料。因此，我们需要建立一个RF的参考材料，即使它是一个临时的。提供RF测量试剂的科学团体（日本流变学学会和日本实验医学会）和制造商应共同努力改善RF测量。
OBJECTIVES
To disclose the current situation of rheumatoid factor (RF) measurement in Japan.
METHOD
A small-scale survey was performed among members of the committee for the improvement of RF measurement in 2003 using manufacturers' RF reference materials valued based on WHO RF reference and pooled sera. A nationwide questionnaire survey was also performed. Questionnaires were sent to 356 educational institutes certified by the Japan College of Rheumatology in December 2004.
RESULTS
The small-scale survey showed inter-laboratory differences of measured RF values, especially using RF references, and less in pooled sera. In the questionnaire survey (187 responses, recovery, 53%), qualitative or semi-quantitative RF measurement methods were used in a small number of institutes and quantitative methods were used in large numbers of institutes, the latex immunoassay (turbidimetric or nepherometric) was predominantly employed. The measuring instruments were various. The upper limit of the reference interval was distributed widely from 5 IU/ml to 40 IU/ml, indicating obvious inter-laboratory differences in RF measurement. Many institutes used the reference intervals recommended by the manufacturers.
CONCLUSION
There are remarkable inter-laboratory differences in RF measurement. Clearly, one of the major issues is the lack of reference materials. We therefore need to establish a reference material of RF, even if it is a temporary one. Scientific societies (Japan College of Rheumatology and Japanese Society of Laboratory Medicine) and manufacturers providing RF measurement reagents should work together for the improvement of RF measurement.