Redefining the Progression of Lineage Segregations during Mammalian Embryogenesis by Clonal Analysis
Redefining the Progression of Lineage Segregations during Mammalian Embryogenesis by Clonal Analysis
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DOI：
10.1016/j.devcel.2009.08.002
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发表时间：
2009-09-15
影响因子：
11.8
通讯作者：
Nicolas, Jean-Francois
中科院分区：
文献类型：
--
作者：
Tzouanacou, Elena;Wegener, Amelie;Nicolas, Jean-Francois
克隆谱系信息是揭示细胞命运选择的基础。利用遗传单细胞标记技术，我们研究了小鼠前后轴形成过程中的谱系分离。我们发现，虽然内胚层和表面外胚层分离原肠胚形成过程中，神经外胚层和中胚层共享一个共同的祖先坚持通过轴伸长的所有阶段。这些数据挑战了原肠胚形成形成的三个胚层构成多能外胚层向组织特异性前体分化的主要分支点的范式。双能神经中胚层祖细胞显示自我更新的特性，并可能代表细胞基质耦合持续的轴向伸长和协调这些组织的分化。这些发现对于解释几种小鼠突变体的表型缺陷和胚胎干细胞体外定向分化具有重要意义。
Clonal lineage information is fundamental in revealing cell fate choices. Using genetic single-cell labeling in utero, we investigated lineage segregations during anteroposterior axis formation in mouse. We show that while endoderm and surface ectoderm segregate during gastrulation, neural ectoderm and mesoderm share a common progenitor persisting through all stages of axis elongation. These data challenge the paradigm that the three germ layers, formed by gastrulation, constitute the primary branchpoints in differentiation of the pluripotent epiblast toward tissue-specific precursors. Bipotent neuromesodermal progenitors show self-renewing characteristics and may represent the cellular substrate coupling sustained axial elongation and coordinated differentiation of these tissues. These findings have important implications for the interpretation of the phenotypic defects of several mouse mutants and the directed differentiation of embryonic stem (ES) cells in vitro.