CHARACTERIZATION AND PROPERTIES OF THE HUMAN AND BOVINE LACTOTRANSFERRINS EXTRACTED FROM THE FAECES OF NEWBORN INFANTS
CHARACTERIZATION AND PROPERTIES OF THE HUMAN AND BOVINE LACTOTRANSFERRINS EXTRACTED FROM THE FAECES OF NEWBORN INFANTS
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DOI：
10.1111/j.1651-2227.1982.tb09560.x
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发表时间：
1982-11
期刊：
影响因子：
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通讯作者：
G. Spik;B. Brunet;C. Mazurier-Dehaine;G. Fontaine;J. Montreuil
中科院分区：
文献类型：
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作者：
G. Spik;B. Brunet;C. Mazurier-Dehaine;G. Fontaine;J. Montreuil
摘要。母乳喂养的婴儿粪便提取物中的人乳转铁蛋白的特点。从出生到3周的人乳转铁蛋白定量测定显示，每日消除量从35 mg降至5 mg。对应于内源性分泌的共乳转铁蛋白的量计算为0.5至1 mg/天。当给婴儿喂食补充部分或完全铁饱和的人乳或牛乳转铁蛋白的牛奶饮食时，排泄的粪乳转铁蛋白的量取决于乳转铁蛋白的来源和铁饱和度。特别是，粪便中牛共乳转铁蛋白的量平均为200 mg/天。通过离子交换层析或亲和层析分离人和牛共乳转铁蛋白，并且仍然能够结合铁。婴儿胃或十二指肠分泌物体外水解的天然人和牛乳转铁蛋白的指纹图谱表明，这两种蛋白质都没有被广泛消化。这表明婴儿摄入的这些乳转铁蛋白没有被完全破坏，并保持其结合铁的能力，因此可以补充肠道中内源性乳转铁蛋白的抑菌作用。
ABSTRACT. Human lactotransferrin was characterized in the faecal extracts from breastfed babies. Quantitative determination of human copro‐lactotransferrin from birth for a period of up to 3 weeks showed that the daily elimination decreased from 35 to 5 mg. The amount of copro‐lactotransferrin corresponding to the endogenous secretion was calculated to be from 0.5 to 1mg per day. When a cow's milk diet supplemented by partially or completely iron‐saturated human or bovine lactotransferrin was fed to the babies, the amounts of coprolactotransferrin excreted depended on the origin and on the iron saturation of the lactoransferrin. In particular, the amount of bovine copro‐lactotransferrin in the faeces averaged 200 mg per day. The human and bovine copro‐lactotransferrins were isolated by ion‐exchange chromatography or by affinity chromatography and were still able to bind iron. The fingerprints of native human and bovine lactotransferrins hydrolysed in vitro by infant's gastric or duodenal secretions showed that both proteins were not extensively digested. This demonstrates that these lactotransferrins ingested by babies are not completely destroyed and keep their ability to bind iron, and thus may supplement the bacteriostatic effects of the endogenous lactotransferrin in the intestinal tract.