Jasmonic Acid Enhances Al-Induced Root Growth Inhibition
Jasmonic Acid Enhances Al-Induced Root Growth Inhibition
复制标题
茉莉酸增强铝诱导的根生长抑制作用。
DOI：
10.1104/pp.16.01756
复制
发表时间：
2017
期刊：
影响因子：
7.4
通讯作者：
Ding Zhaojun
中科院分区：
文献类型：
--
作者：
Yang Zhong Bao;He Chunmei;Ma Yanqi;Herde Marco;Ding Zhaojun
植物激素如乙烯、生长素等参与了铝诱导的根生长抑制的调控。虽然茉莉酸（JA）已被报道通过与乙烯和生长素的相互作用在响应环境胁迫的根生长和发育的调节中起关键作用，但其在响应铝胁迫的根生长调节中的作用尚不清楚。在试图阐明JA的作用，我们发现，外源JA的应用增强铝诱导的根生长抑制。此外，与JA生物合成或信号转导缺陷的突变体的表型分析表明，JA参与铝诱导的根生长抑制的调节。JA受体CORONATINE INSENSITIVE 1（COI 1）和JA信号调节子MYC 2的表达在Al胁迫下上调。这一过程与COI 1介导的铝诱导的根生长抑制在铝胁迫下控制乙烯，而不是生长素。转录组学分析表明，许多铝胁迫下的应答基因受到JA信号的调控。野生型和coi 1 - 2突变体之间微管组织相关基因的差异反应与铝胁迫下coi 1中皮层微管解聚的变化相一致。此外，ALMT介导的苹果酸分泌，从而铝排斥从根响应铝胁迫也受到COI 1介导的JA信号。总之，这项研究表明，根生长抑制是由COI 1介导的JA信号独立于生长素信号，并提供了新的见解植物生长素介导的根生长抑制响应铝胁迫。
Phytohormones such as ethylene and auxin are involved in the regulation of the aluminum (Al)-induced root growth inhibition. Although jasmonate (JA) has been reported to play a crucial role in the regulation of root growth and development in response to environmental stresses through interplay with ethylene and auxin, its role in the regulation of root growth response to Al stress is not yet known. In an attempt to elucidate the role of JA, we found that exogenous application of JA enhanced the Al-induced root growth inhibition. Furthermore, phenotype analysis with mutants defective in either JA biosynthesis or signaling suggests that JA is involved in the regulation of Al-induced root growth inhibition. The expression of the JA receptor CORONATINE INSENSITIVE1 (COI1) and the key JA signaling regulator MYC2 was up-regulated in response to Al stress in the root tips. This process together with COI1-mediated Al-induced root growth inhibition under Al stress was controlled by ethylene but not auxin. Transcriptomic analysis revealed that many responsive genes under Al stress were regulated by JA signaling. The differential responsive of microtubule organization-related genes between the wild-type andcoi1-2mutant is consistent with the changed depolymerization of cortical microtubules incoi1under Al stress. In addition, ALMT-mediated malate exudation and thus Al exclusion from roots in response to Al stress was also regulated by COI1-mediated JA signaling. Together, this study suggests that root growth inhibition is regulated by COI1-mediated JA signaling independent from auxin signaling and provides novel insights into the phytohormone-mediated root growth inhibition in response to Al stress.