Relationship between the activation of cyclic AMP responsive element binding protein and ischemic tolerance in the penumbra region of rat cerebral cortex
Relationship between the activation of cyclic AMP responsive element binding protein and ischemic tolerance in the penumbra region of rat cerebral cortex
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DOI：
10.1016/s0304-3940(02)00752-8
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发表时间：
2002-10-04
影响因子：
2.5
通讯作者：
Kawahara, K
中科院分区：
文献类型：
--
作者：
Nakajima, T;Iwabuchi, S;Kawahara, K
已知应用短暂的缺血，即大脑中动脉区域的预处理治疗，可以产生缺血耐受性，减少致死性缺血后半暗带区域的脑梗塞体积。然而，人们对预处理诱导缺血耐受的分子机制知之甚少。在本研究中，我们通过免疫组织化学和蛋白质印迹检查了预处理和非预处理大鼠局灶性脑缺血1小时后环AMP反应元件结合蛋白（CREB）磷酸化模式的差异。缺血1小时后，预处理大鼠的半影区CREB磷酸化比未预处理大鼠更快增强。结果表明，半影区 CREB 磷酸化的立即增强阻止了预处理大鼠中的梗塞扩散。 (C) 2002 Elsevier Science Ireland Ltd. 保留所有权利。
Application of a brief period of ischemia, i.e. preconditioning treatment of the middle cerebral artery territory, has been known to produce ischemic tolerance, reducing cerebral infarction volume in the penumbra region after lethal ischemia. However, little is known about the molecular mechanisms responsible for preconditioning-induced ischemic tolerance. In the present study, we examined the difference in the phosphorylation pattern of cyclic AMP responsive element binding protein (CREB) after 1 h of focal cerebral ischemia between preconditioned and non-preconditioned rats by immunohistochemistry and Western blotting. The phosphorylation of CREB in the penumbra region was more rapidly enhanced in the preconditioned rats than in the non-preconditioned rats after 1 h of ischemia. The result suggested that the immediate enhancement in the phosphorylation of CREB in the penumbra region prevented the spread of infarction in the preconditioned rats. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.