RESTRICTION FRAGMENT VARIATION IN THE NUCLEAR AND CHLOROPLAST GENOMES OF CULTIVATED AND WILD SORGHUM-BICOLOR
RESTRICTION FRAGMENT VARIATION IN THE NUCLEAR AND CHLOROPLAST GENOMES OF CULTIVATED AND WILD SORGHUM-BICOLOR
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DOI：
10.1007/bf00222873
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发表时间：
1992-11-01
影响因子：
5.4
通讯作者：
DOEBLEY, J
中科院分区：
文献类型：
--
作者：
ALDRICH, PR;DOEBLEY, J
使用 50 个低拷贝数核 DNA 序列探针检测限制性片段长度多态性 (RFLP)，对 56 个栽培和野生高粱种质的遗传多样性进行了调查。这些探针揭示了野生高粱比栽培高粱具有更大的遗传多样性，包括野生高粱中每个基因座的等位基因数量更多以及多态性基因座的比例更大。与之前发表的相同种质的同工酶分析相比，RFLP 分析揭示了每个位点的等位基因数量更多。此外，许多 RFLP 等位基因的频率在 0.25-0.75 之间，而绝大多数同工酶等位基因要么罕见（< 0.25），要么接近固定（> 0.75）。使用 RFLP 计算时，种质间遗传距离和地理距离之间的相关性比使用同工酶数据计算的相关性更强。核和叶绿体限制位点分析揭示的系统关系表明栽培高粱源自野生高粱。芦竹。野生基因库中与栽培品种基因最相似的部分来自非洲中部东北部。先前公布的数据还表明，这很可能是高粱驯化的主要地区。野生高粱和栽培高粱之间的基因渗入是根据核和叶绿体 DNA 标记显示的不一致关系推断出来的。基因渗入显然很少发生，因此作物及其野生近缘种保持着不同的遗传组成。
Fifty-six accessions of cultivated and wild sorghum were surveyed for genetic diversity using 50 low-copy-number nuclear DNA sequence probes to detect restriction fragment length polymorphisms (RFLPs). These probes revealed greater genetic diversity in wild sorghum than in cultivated sorghum, including a larger number of alleles per locus and a greater portion of polymorphic loci in wild sorghum. In comparison to previously published isozyme analyses of the same accessions, RFLP analysis reveals a greater number of alleles per locus. Furthermore, many RFLP alleles have frequencies between 0.25-0.75, while the vast majority of isozyme alleles are either rare (< 0.25) or near fixation (> 0.75). Correlations between genetic and geographic distances among the accessions were stronger when calculated with RFLP than with isozyme data. Systematic relationships revealed by nuclear and chloroplast restriction site analysis indicate that cultivated sorghum is derived from the wild ssp. arundinaceum. The portion of the wild gene pool most genetically similar to the cultivars is from central-northeastern Africa. Previous published data also suggested that this is most likely the principal area of domestication of sorghum. Introgression between wild and cultivated sorghum was inferred from disconcordant relationships shown by nuclear and chloroplast DNA markers. Introgression apparently occurs infrequently enough that the crop and its wild relatives maintain distinct genetic constitutions.