Intracellular calcium reaches different levels of elevation in hyperactivated and acrosome-reacted hamster sperm.
Intracellular calcium reaches different levels of elevation in hyperactivated and acrosome-reacted hamster sperm.
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在过度活跃和顶体反应的仓鼠精子中，细胞内钙达到不同程度的升高。
DOI：
10.1002/mrd.1080420310
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Dai,X
中科院分区：
文献类型：
--
作者：
Suarez,SS;Dai,X
钙在精子运动、过度激活和顶体反应中起着重要作用，但这两种状态下的胞浆钙水平之间的关系尚不清楚。在两组实验中，使用了钙离子指示剂Indo-1来检测运动仓鼠精子中的钙离子。在第一个实验中，对激活的、过度激活的和透明带诱导的顶体反应/过度激活的精子在每种状态的活动峰值时间进行了分析。在第二个实验中，在一个时间点上分析了所有状态下的精子。两组精子顶体区、顶体后区和鞭毛中段的平均钙离子浓度在过度激活的精子中高于激活的精子，在顶体反应/过度激活的精子中高于未反应/过度激活的精子(P≤0.001)。在过度激活的精子中，精子中部的钙离子浓度比头部的增加幅度更大，而顶体反应的精子则相反。鞭毛节拍周期频率的振荡主要在顶体反应的精子的近端鞭毛中段检测到，正如以前报道的那样，它们发生在激活和过度激活的精子中。因此，精子中的钙离子可能维持在两种不同的升高水平，并在顶体反应后继续振荡。©1995 Wiley-Liss公司
Calcium plays a role in sperm motility hyperactivation and the acrosome reaction, but the relationship between cytoplasmic calcium (Ca2+in) levels in the two states was heretofore unknown. The Ca2+indicator indo‐1 was used to detect Ca2+inin moving hamster sperm in two sets of experiments. In the first experiment, activated, hyperactivated, and zona pellucida‐induced acrosome‐reacted/hyperactivated sperm were analyzed at the time of peak of activity for each state. In the second experiment, sperm in all states were analyzed at one time point. In both sets, mean Ca2+inin the acrosomal region, postacrosomal region, and flagellar midpiece was greater in hyperactivated sperm than in activated sperm, and in acrosome‐reacted/hyperactivated sperm than in unreacted/hyperactivated sperm (P ≤ 0.001). Ca2+inhad increased to a greater extent in the midpiece than in the head in hyperactivated sperm, while the reverse was true for acrosome‐reacted sperm. Oscillations at the frequency of the flagellar beat cycle were detected chiefly in the proximal flagellar midpiece of acrosome‐reacted sperm, as they had been previously reported to occur in activated and hyperactivated sperm. Thus, Ca2+inmay be maintained at two different elevated levels in sperm, and continues to oscillate after the acrosome reaction. © 1995 wiley‐Liss, Inc.