QUANTITATION OF HIV-1 RNA IN PLASMA PREDICTS OUTCOME AFTER SEROCONVERSION
QUANTITATION OF HIV-1 RNA IN PLASMA PREDICTS OUTCOME AFTER SEROCONVERSION
复制标题
DOI：
10.7326/0003-4819-122-8-199504150-00003
复制
发表时间：
1995-04-15
影响因子：
39.2
通讯作者：
GUPTA, P
中科院分区：
文献类型：
--
作者：
MELLORS, JW;KINGSLEY, LA;GUPTA, P
目的：探讨血浆中人类免疫缺陷病毒 1 型 (HIV-1) RNA 的数量与获得性免疫缺陷综合征 (AIDS) 或血清转化后 CD4+ T 细胞计数下降的风险之间的关系。设计：前瞻性研究。患者：62 名记录有 HIV-1 血清转化的同性恋男性。地点：大学门诊环境。测量：临床状态、CD4+ T 细胞计数以及血浆和血清样本每 6 个月获得一次。使用分支 DNA (bDNA) 测定法对血浆中的人类免疫缺陷病毒 RNA 进行定量。检测血清样本中的新蝶呤、β(2)-微球蛋白和免疫复合物解离的 HIV-1 p24 抗原。结果：62 名男性中有 18 名 (29%) 患上艾滋病； 21 人 (34%) 在没有艾滋病的情况下 CD4+ T 细胞计数显着下降； 23 名 (37%) 的 CD4+ T 细胞计数稳定。对于每个参与者，HIV-1 RNA结果被分为四组之一：1)所有样品中HIV-1 RNA的检测(>1 x 10(4)基因组当量/mL。[Eq/mL])； 2）在大部分样品中检测到（大于或等于50%）； 3) 检测率小于50%的样品； 4) 所有样品均未检测到。所有或大多数样本中 HIV-1 RNA 的检测与 AIDS（18 名患者中的 16 名）以及 CD4+ T 细胞计数下降（21 名患者中的 13 名）与稳定的 CD4+ T 细胞计数（23 名患者中的 4 名；P < 0.001）密切相关，相反，HIV-1 RNA 的缺失（
Objective: To investigate the relation between the quantity of human immunodeficiency virus type 1 (HIV-1) RNA in plasma and the risk for the acquired immunodeficiency syndrome (AIDS) or a decline in the CD4+ T-cell count after seroconversion.Design: Prospective study.Patients: 62 homosexual men with documented HIV-1 seroconversion.Setting: University outpatient setting.Measurements: Clinical status, CD4+ T-cell counts, and plasma and serum samples were obtained every 6 months. Human immunodeficiency virus RNA in plasma was quantitated with a branched-DNA (bDNA) assay. Serum samples were assayed for neopterin, beta(2)-microglobulin, and immune complex dissociated HIV-1 p24 antigen.Results: 18 of 62 (29%) men developed AIDS; 21 (34%) had a significant decline in the CD4+ T-cell count without AIDS; and 23 (37%) had a stable CD4+ T-cell count. For each participant, HIV-1 RNA results were categorized into one of four groups: 1) detection of HIV-I RNA(>1 x 10(4) genome equivalents/mL. [Eq/mL]) in all samples; 2) detection in most samples (greater than or equal to 50%); 3) detection in fewer than 50% of samples; and 4) detection in none of the samples. Detection of HIV-1 RNA in all or most samples was strongly associated with AIDS (16 of 18 patients) and a decline in the CD4+ T-cell count (13 of 21 patients) compared with a stable CD4+ T-cell count (4 of 23 patients; P < 0.001), Conversely, the absence of HIV-1 RNA(