2-DIMENSIONAL CRYSTALS OF STREPTAVIDIN ON BIOTINYLATED LIPID LAYERS AND THEIR INTERACTIONS WITH BIOTINYLATED MACROMOLECULES
2-DIMENSIONAL CRYSTALS OF STREPTAVIDIN ON BIOTINYLATED LIPID LAYERS AND THEIR INTERACTIONS WITH BIOTINYLATED MACROMOLECULES
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DOI：
10.1016/s0006-3495(91)82232-9
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发表时间：
1991-02-01
影响因子：
3.4
通讯作者：
KORNBERG, RD
中科院分区：
文献类型：
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作者：
DARST, SA;AHLERS, M;KORNBERG, RD
当链霉亲和素与空气/水界面处的生物素化脂质层特异性结合时，会形成二维晶体。 通过电子晶体学从晶体确定的链霉亲和素的三维结构与通过X射线晶体学确定的结构很好地对应。 电子和X射线晶体结构的比较揭示了面向水溶液的二维晶体表面上存在游离生物素结合位点。 游离的生物素结合位点可以用生物素化铁蛋白特异性标记。 链霉亲和素/生物素化脂质系统可以提供形成生物素化大分子二维晶体的通用方法。
Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography. Comparison of the electron and x-ray crystallographic structures reveals the occurrence of free biotin-binding sites on the surface of the two-dimensional crystals facing the aqueous solution. The free biotin-binding sites could be specifically labeled with biotinylated ferritin. The streptavidin/biotinylated lipid system may provide a general approach for the formation of two-dimensional crystals of biotinylated macromolecules.