Human mast cells release Interleukin-8 and induce neutrophil chemotaxis on contact with activated T cells
Human mast cells release Interleukin-8 and induce neutrophil chemotaxis on contact with activated T cells
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DOI：
10.1111/j.1398-9995.2005.00886.x
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发表时间：
2005-10-01
期刊：
影响因子：
12.4
通讯作者：
Mekori, YA
中科院分区：
文献类型：
--
作者：
Salamon, P;Shoham, NG;Mekori, YA
背景：最近显示，肥大细胞通过 TNF-α 和 MIP-2（人 IL-8 的功能性鼠类类似物）在体内 T 细胞介导的皮肤 DTH 反应过程中控制中性粒细胞的募集。虽然激活肥大细胞的 T 细胞传递的信号的性质尚未确定，但正如之前报道的那样，我们假设这两个细胞群之间存在直接串扰（即异型粘附）。目的：本研究旨在深入了解肥大细胞-T 细胞接触在 IL-8 表达和释放中的功能作用，及其对中性粒细胞趋化性的影响。方法：通过 Affymetrix 鉴定 IL-8 基因表达GeneChip 阵列，通过 RT-PCR 进行验证，并通过 ELISA 测量蛋白质。通过使用改良的博伊登室测定来评估趋化性。结果：与静息T细胞相比，发现肥大细胞在与从活化获得的膜一起孵育时表达和释放显着更高浓度的IL-8。从这些活化的肥大细胞获得的上清液诱导显着的中性粒细胞趋化性，该趋化性可通过中和 IL-8 mAb 来抑制。结论：因此，活化的 T 细胞在与肥大细胞异型粘附时，传递必要的信号，使肥大细胞释放细胞迁移到抗原攻击部位所需的细胞因子和趋化因子，从而促进 T 细胞介导的炎症过程。
Background: Mast cells have recently been shown to control neutrophil recruitment during T-cell mediated cutaneous DTH reaction in vivo through TNF-alpha and MIP-2, the functional murine analogue of human IL-8. Although the nature of signals transmitted from T cells which activate mast cells has not yet been defined, we hypothesized that a direct cross-talk (i.e. heterotypic adhesion) between these two cell populations exists, as has previously been reported.Aims: The present study was aimed at gaining insight into the functional role of mast cell-T cell contact in expression and release of IL-8, and its effect on neutrophil chemotaxis.Methods: The IL-8 gene expression was identified by Affymetrix GeneChip arrays, validated by RT-PCR and the protein measured by ELISA. Chemotaxis was evaluated by using a modified Boyden chamber assay.Results: Mast cells were found to express and release significantly higher concentrations of IL-8 on incubation with membranes obtained from activated, as compared to resting T cells. Supernatants obtained from these activated mast cells induced significant neutrophil chemotaxis that was inhibited by neutralizing mAb to IL-8.Conclusions: Thus, activated T cells, on heterotypic adhesion to mast cells, deliver the necessary signals for the latter to release cytokines and chemokines necessary for cell migration to sites of antigen challenge, thereby facilitating T-cell mediated inflammatory processes.