Comparative glycoprofiling of HIV gp120 immunogens by capillary electrophoresis and MALDI mass spectrometry.
Comparative glycoprofiling of HIV gp120 immunogens by capillary electrophoresis and MALDI mass spectrometry.
复制标题
通过毛细管电泳和 MALDI 质谱法对 HIV gp120 免疫原进行比较糖谱分析。
DOI：
10.1002/elps.201500054
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发表时间：
2015
期刊：
影响因子：
2.9
通讯作者：
Guttman,András
中科院分区：
文献类型：
--
作者：
Guttman,Miklós;Váradi,Csaba;Lee,KellyK;Guttman,András
人类免疫缺陷病毒（HIV）包膜糖蛋白（Env）是病毒表面的主要抗原特征，在HIV疫苗学中至关重要。用Env的gp 120亚基进行的疫苗试验正在进行中，最近的RV 144试验显示出中等疗效。gp 120被N-连接聚糖密集覆盖，被认为有助于逃避宿主的体液免疫反应。为了评估gp 120构建体之间的整体糖基化模式如何变化，通过CE结合LIF检测和MALDI-MS检查了几种gp 120的聚糖谱。糖基化配置文件被认为是类似的慢性与发射机/创始人分离株和不同的进化枝之间的gp 120只略有不同。这项研究表明，添加特定的标签，如RV 144试验中使用的单纯疱疹病毒糖蛋白D标签，对整体糖基化模式有显着影响。这种效应可能会影响各种Env免疫原的免疫原性，并应考虑用于未来的疫苗策略，强调本文所述的糖基化分析方法的重要性。
The human immunodeficiency virus (HIV) envelope glycoprotein (Env) is the primary antigenic feature on the surface of the virus and is of key importance in HIV vaccinology. Vaccine trials with the gp120 subunit of Env are ongoing, with the recent RV144 trial showing moderate efficacy. gp120 is densely covered withN‐linked glycans that are thought to help evade the host's humoral immune response. To assess how the global glycosylation patterns vary between gp120 constructs, the glycan profiles of several gp120s were examined by CE with LIF detection and MALDI‐MS. The glycosylation profiles were found to be similar for chronic versus transmitter/founder isolates and only varied moderately between gp120s from different clades. This study revealed that the addition of specific tags, such as the herpes simplex virus glycoprotein D tag used in the RV144 trial, had significant effects on the overall glycosylation patterns. Such effects are likely to influence the immunogenicity of various Env immunogens and should be considered for future vaccine strategies, emphasizing the importance of the glycosylation analysis approach described in this paper.