Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG.
Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG.
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DOI：
10.1002/vms3.121
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发表时间：
2018-11
影响因子：
1.7
通讯作者：
Otoi T
中科院分区：
文献类型：
--
作者：
Hirata M;Tanihara F;Taniguchi M;Takagi M;Terazono T;Otoi T
狗的卵泡动力学还不是很清楚。卵巢的成像在技术上是困难的；然而，夹在皮下部位的卵巢可以更容易地使用超声波成像进行监测。本研究利用超声成像技术对夹在皮下部位的卵巢进行超声成像，研究了激素治疗对犬卵巢卵泡发育的影响。皮下注射500兆单位的马绒毛膜促性腺激素(CG)和1000兆单位的人绒毛膜促性腺激素(HCG)诱导发情。5只母犬在给药后11天给药1000IU hCG。每天使用7.5 MHz扇形换能器进行卵巢超声检查、阴道细胞学检查以及血清雌激素和孕酮测定，直到注射CG/hCG后20天。对其中两只母犬的阴道分泌物和阴道细胞学进行观察。给药后2~8天，所有母犬均可观察到新的卵泡生长(直径1.0 mm)。平均每个卵巢卵泡直径2.8~5.5 mm，最大卵泡数4~16个。给药后雌激素值升高，其中3只雌孕酮浓度升高(>；2 ng/mL−-1)。但直到注射hCG后第9天，卵泡才排卵。总而言之，尽管被检查的母犬数量有限，但可以使用超声成像来监测夹在皮下部位的卵巢中的卵泡生长。卵巢超声检查显示，注射ECG/hCG可诱导新的卵泡生长，注射hCG可引起雌激素浓度升高，但不能促进排卵。
Ovarian follicular dynamics is not well known in dogs. Imaging of ovaries is technically difficult; however, ovaries clamped at a subcutaneous site can more easily be monitored using ultrasound imaging. This study investigated the follicular development of canine ovaries stimulated by hormone treatment using ultrasound imaging of the ovaries clamped at a subcutaneous site. Oestrus was induced using subcutaneous administration of 500 IU equine chorionic gonadotropin (eCG) and 1000 IU human chorionic gonadotropin (hCG) (eCG/hCG). Five bitches were given 1000 IU hCG 11 days after eCG/hCG administration. Examinations with ovarian ultrasonography using a 7.5‐MHz sector transducer, vaginal cytology, and assays of serum oestrogen and progesterone were performed daily until 20 days after eCG/hCG administration. Serosanguineous vaginal discharges and vaginal cytology of two of the bitches were observed. New follicular growth (>1.0 mm in diameter) was observed in all bitches from 2 to 8 days after eCG/hCG administration. The mean diameter of follicles and maximum numbers of follicles per ovary ranged from 2.8 to 5.5 mm and 4 to 16, respectively. The elevation in oestrogen concentrations after eCG/hCG administration was observed in all bitches, and elevation in progesterone concentration (>2 ng mL−1) was observed in three bitches. However, no follicles ovulated until 9 days after hCG administration. In conclusion, although the number of examined bitches were limited, follicular growth in ovaries clamped at a subcutaneous site can be monitored using ultrasound imaging. Ovarian ultrasonography showed that eCG/hCG administration induced new follicular growth and hCG administration induced increases in oestrogen concentrations but not ovulation by hCG administration.
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DOI：
10.1530/jrf.0.0930443
发表时间：
1991-11-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
ENGLAND, GCW;ALLEN, WE
通讯作者：
ALLEN, WE
影响因子：
2.8
作者：
Stornelli, M. C.;Garcia Mitacek, M. C.;Stornelli, M. A.
通讯作者：
Stornelli, M. A.
影响因子：
1.1
作者：
WRIGHT, PJ
通讯作者：
WRIGHT, PJ
影响因子：
1.7
作者：
England, G. C. W.;Russo, M.;Freeman, S. L.
通讯作者：
Freeman, S. L.
影响因子：
2.8
作者：
Kutzler, M. A.
通讯作者：
Kutzler, M. A.