Simple and Universal Platform for Logic Gate Operations Based on Molecular Beacon Probes
Simple and Universal Platform for Logic Gate Operations Based on Molecular Beacon Probes
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DOI：
10.1002/smll.201102758
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发表时间：
2012-07-23
期刊：
影响因子：
13.3
通讯作者：
Park, Hyun Gyu
中科院分区：
文献类型：
--
作者：
Park, Ki Soo;Seo, Myung Wan;Park, Hyun Gyu
本文描述了一种新的平台技术，利用该技术以发夹结构的分子信标探针作为基本工作单元构建分子逻辑门。在这个逻辑门操作系统中，单链DNA被用作输入，通过序列特异性相互作用诱导分子信标探针的构象变化。然后将分子信标探针打开产生的荧光信号用作输出读数。重要的是，由于逻辑门基于 DNA，因此可以保持输入/输出同质性，因此可以通过组合单独操作的逻辑门或将一个门的 DNA 输出设计为另一门的输入来实现将它们连接到多层电路中。通过这种新颖的策略，在分子水平上成功构建了一套完整的二输入逻辑门，包括OR、AND、XOR、INHIBIT、NOR、NAND、XNOR和IMPLICATION。本文开发的逻辑门可以可逆地操作，以通过施加附加输入或移除链来执行设置-重置功能。总之，这些结果引入了一种用于逻辑门操作的新平台技术，该技术能够实现各种计算元件之间复杂通信所需的高阶电路。
A new platform technology is herein described with which to construct molecular logic gates by employing the hairpin-structured molecular beacon probe as a basic work unit. In this logic gate operation system, single-stranded DNA is used as the input to induce a conformational change in a molecular beacon probe through a sequence-specific interaction. The fluorescent signal resulting from the opening of the molecular beacon probe is then used as the output readout. Importantly, because the logic gates are based on DNA, thus permitting input/output homogeneity to be preserved, their wiring into multi-level circuits can be achieved by combining separately operated logic gates or by designing the DNA output of one gate as the input to the other. With this novel strategy, a complete set of two-input logic gates is successfully constructed at the molecular level, including OR, AND, XOR, INHIBIT, NOR, NAND, XNOR, and IMPLICATION. The logic gates developed herein can be reversibly operated to perform the set-reset function by applying an additional input or a removal strand. Together, these results introduce a new platform technology for logic gate operation that enables the higher-order circuits required for complex communication between various computational elements.