Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia
Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia
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DOI：
10.1099/0022-1317-49-12-1075
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发表时间：
2000-12-01
影响因子：
3
通讯作者：
Sirisinha, S
中科院分区：
文献类型：
--
作者：
Anuntagool, N;Naigowit, P;Sirisinha, S
采用基于单克隆抗体的乳胶凝集 (MAb-LA) 测试快速鉴定社区获得性败血症患者血培养液中的类鼻疽伯克霍尔德氏菌。这些患者被收治在泰国东北部的 12 家医院，该地区是类鼻疽流行地区。收集血样并立即添加到血培养瓶中，在自动（五家医院）或手动（七家医院）培养系统中孵育。在总共 1369 份培养阳性标本中，204 份标本为假鼻疽 B 培养阳性，其中 194 份 (95%) MAb-LA 呈阳性，并且血培养系统的类型不影响阳性率。与培养检测和生化测试确认相比，MAb-LA 测试对这些标本的性能非常令人满意，灵敏度为 95.1%，特异性为 99.7%，阳性和阴性预测值分别为 98.8% 和 99.2%。所描述的方法具有高度可重复性，即使没有经验的实验室人员也可以简单地执行，并且不需要昂贵或复杂的设备。
A monoclonal antibody-based latex agglutination (MAb-LA) test was employed for the rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. These patients were admitted to 12 hospitals in the northeastern part of Thailand which is a region known to be endemic for melioidosis. Blood samples were collected and immediately added to the blood culture bottles which were incubated in either automated (five hospitals) or manual (seven hospitals) culture systems. Of a total of 1369 culture-positive specimens, 204 specimens were culture-positive for B, pseudomallei Of those, 194 (95%) were positive by MAb-LA and the type of blood culture system did not affect positivity rates. The performance of the MAb-LA test on these specimens was highly satisfactory compared with culture detection and confirmation by biochemical test,,vith 95.1% sensitivity, 99.7% specificity and 98.8% and 99.2% for positive and negative predictive values, respectively. The method described is highly reproducible, simple to perform even by inexperienced laboratory personnel and does not require expensive or elaborate equipment.