Exploitation of Parthenium hysterophorous biomass as low-cost substrate for cellulase and xylanase production under solid- state fermentation using Talaromyces stipitatus MTCC 12687
Exploitation of Parthenium hysterophorous biomass as low-cost substrate for cellulase and xylanase production under solid- state fermentation using Talaromyces stipitatus MTCC 12687
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DOI：
10.1016/j.jrras.2018.01.003
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发表时间：
2018-10-01
影响因子：
1.7
通讯作者：
Dutt, Dharm
中科院分区：
文献类型：
--
作者：
Bharti, Amit Kumar;Kumar, Amit;Dutt, Dharm
为了使酶的生产过程在SSF方面相当便宜，使用经济的底物是非常感兴趣的。在所用的8种固体基质中，银胶菊的生物量表现出其潜力，并显示出最大的内切葡聚糖酶（30 IU/gds）、木聚糖酶（582 IU/gds）和FPase（2.86 FPU/gds）活性。在优化培养条件的过程中，Talaromyces stipitatus MTCC 12687产内切葡聚糖酶最高（53.3 IU/gds），FPase（4.51 FPU/gds）和b葡萄糖苷酶（62.6 IU/gds），温度下（30 ℃），孵育时间（第5天），以蛋白胨为氮源，Tween-80为表面活性剂，在SSF条件下，pH值为6.0，水分含量为80%。在优化的过程中，不同的酶活性，即内切葡聚糖酶，木聚糖酶，FPase和β-葡萄糖苷酶分别提高了约2.0，1.54，2.0和2.7倍，与它们各自的对照相比。在水解48小时后，Talaromyces stipitatus MTCC 12687酶水解未处理的和有机溶剂预处理的P. heterophorous生物质，分别释放93 +/- 3.87和734 +/- 17 mg/g的还原糖。(C)2018年埃及辐射科学与应用学会。由爱思唯尔公司制作和主持
To make the enzyme production process appreciably inexpensive in respect of SSF, use of economical substrates is of great interest. Out of total eight employed solid substrate, Parthenium hysterophorous biomass exhibited its potential and displayed maximum endoglucanase (30 IU/gds), xylanase (582 IU/gds) and FPase (2.86 FPU/gds) activities. During optimization of cultural parameters, Talaromyces stipitatus MTCC 12687 produced maximum endoglucanase (53.3 IU/gds), FPase (4.51 FPU/gds) and bglucosidase (62.6 IU/gds) at temperature (30 degrees C), incubation time (5th day), pH (6.0) and moisture content (80%) with the supplementation of peptone as the nitrogen source and Tween-80 as the surfactant under SSF conditions. During the process of optimization different enzyme activities viz. endoglucanase, xylanase, FPase and beta-glucosidase were enhanced by approximately 2.0, 1.54, 2.0 and 2.7 fold respectively compared to their respective controls. Talaromyces stipitatus MTCC 12687 enzymes hydrolysed untreated and organosolve pretreated P. hysterophorous biomass to release 93 +/- 3.87 and734 +/- 17 mg/g of reducing sugars respectively after hydrolysis time of 48 h. (C) 2018 The Egyptian Society of Radiation Sciences and Applications. Production and hosting by Elsevier B.V.