Ascorbic acid gives different protective effects in human cells exposed to X-rays and heavy ions
Ascorbic acid gives different protective effects in human cells exposed to X-rays and heavy ions
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DOI：
10.1016/j.mrgentox.2010.04.003
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发表时间：
2010-06-17
影响因子：
1.9
通讯作者：
Okayasu, Ryuichi
中科院分区：
文献类型：
--
作者：
Fujii, Yoshihiro;Kato, Takamitsu A.;Okayasu, Ryuichi
本文研究了抗坏血酸作为辐射防护剂的作用及其机理。本文研究了X射线和重离子辐照后正常人成纤维细胞的存活率、DNA双链断裂（DSB）修复和姐妹染色单体交换（SCE）。在平台期（非周期）细胞中用5 mM抗坏血酸照射后处理24 h可增强细胞存活率和DNA双链断裂修复，并降低X射线照射后的SCE。另一方面，在重离子（如碳离子）照射后，仅观察到SCE降低。从我们的数据判断，抗坏血酸的辐射防护作用可能对X射线等非复合型DNA损伤有效。这些发现为重离子辐照引起的DNA损伤和修复机制提供了新的认识。(C)2010 Elsevier BV保留所有权利。
We studied the effects and mechanisms of ascorbic acid as a radiation protector. Cell survival, repair of DNA double strand breaks (DSBs), and sister chromatid exchanges (SCEs) were examined in normal human fibroblasts irradiated with X-rays and heavy ions. Post-irradiation treatment with 5 mM ascorbic acid for 24 h in plateau phase (non-cycling) cells enhanced cell survival and DNA double strand break repair, and reduced SCEs after X-rays irradiation. On the other hand, only reduced SCEs were observed after heavy ion exposure such as to carbon ions.Judging from our data, it is possible that the radioprotective action of ascorbic acid would be effective in non-complex type DNA damage such as induced by X-rays. These findings provide new insight into the mechanism of DNA damage and repair produced by heavy ion irradiation. (C) 2010 Elsevier B.V. All rights reserved.