Islet cryopreservation.
Islet cryopreservation.
复制标题
胰岛冷冻保存。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
N. Kneteman
中科院分区：
文献类型：
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作者：
R. Rajotte;M. Evans;G. Warnock;N. Kneteman
随着人类胰岛分离方法的改进和临床试验的普及，将需要某种形式的储存。冷冻保存将提供许多优点：储存在组织库中以提供足够的胰岛移植给个体受体;调节组织免疫原性;纯化;以及便于从中心到中心的运输。自1976年以来，已经在胰岛冷冻保存方面做了大量工作，据报道，0.025 ℃/min至75 ℃/min的冷却速率是最佳的。在大鼠中，我们已经发现，缓慢冷却到-40摄氏度，并从-196摄氏度快速解冻，可以获得最高的存活率，如体外和体内测量的。该冻融方案还提供了活的胰腺微碎片和纯的狗胰岛，它们都可以诱导延长的正常血糖。这种冷冻程序也延长了胰岛异种移植物的存活时间，并纯化了不需要的外分泌污染物的胰腺微片段。这种冷冻方法现在被用于人类胰岛。低温保存人胰岛对胰岛移植的临床应用有很大的帮助。
As methods for human islet isolation improve and clinical trials become widespread, some form of storage will be needed. Cryopreservation would offer many advantages: storage in a tissue bank to provide sufficient islets to transplant to individual recipients; modulation of tissue immunogenicity; purification; and, facilitate shipment from center to center. Since 1976, much work has been done on islet cryopreservation with cooling rates of 0.025 degrees C/min to 75 degrees C/min being reported as optimum. In rats we have found that slow cooling to -40 degrees C with rapid thawing from -196 degrees C gives the highest survival as measured in-vitro and in-vivo. This freeze-thaw protocol also provides viable pancreatic microfragments and pure dog islets which can both induce prolonged normoglycemia. This freezing procedure also gives prolonged survival of islet xenografts and purifies the pancreatic microfragments of the unwanted exocrine contaminants. This method of freezing is now being used for human islets. Low temperature storage of human islets will greatly help the clinical use of islet transplantation.