Effects of Artesunate on the Expressions of Insulin-Like Growth Factor-1, Osteopontin and C-Telopeptides of Type II Collagen in a Rat Model of Osteoarthritis
Effects of Artesunate on the Expressions of Insulin-Like Growth Factor-1, Osteopontin and C-Telopeptides of Type II Collagen in a Rat Model of Osteoarthritis
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DOI：
10.1159/000479160
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发表时间：
2018-01-01
期刊：
影响因子：
3.1
通讯作者：
Qiang, Bo
中科院分区：
文献类型：
--
作者：
Bai, Zhe;Guo, Xiao-Hui;Qiang, Bo
目的：本研究旨在探讨青蒿琥酯对骨关节炎（OA）大鼠血清、滑液（SF）和软骨组织中胰岛素样生长因子-1（IGF-1）、骨桥蛋白（OPN）和II型胶原C端肽（CTX-II）的影响。方法：建立OA模型。正常模型组、青蒿琥酯组、维三醇S组各20只。采用酶联免疫吸附试验、免疫组化染色和实时定量聚合酶链反应测定大鼠血清、SF和软骨组织中IGF-1、OPN和CTX-II的水平。采用Mankin评分法和苏木精-伊红染色法观察软骨组织的病理变化。研究结果：与正常组比较，模型组血清和SF中IGF-1水平升高，OPN、CTX-II水平降低，软骨组织中则相反。青蒿琥酯组和维三醇S组治疗2周后血清和SF中IGF-1水平逐渐升高，OPN和CTX-Ⅱ水平逐渐下降。模型组病理改变最明显，Mankin评分最高。青蒿琥酯组和Viatril-S组软骨组织中IGF-1水平升高，OPN、CTX-Ⅱ水平降低，而模型组无明显变化。结论：青蒿琥酯和Viatril-S通过升高IGF-1水平，降低OPN和CTX-II水平抑制OA的发生发展。(C)2017 S. Karger AG，巴塞尔
OBJECTIVE: The study aims to explore the effects of artesunate on insulin-like growth factor-1 (IGF-1), Osteopontin (OPN), and C-telopeptides of type II collagen (CTX-II) in serum, synovial fluid (SF), and cartilage tissues of rats with osteoarthritis (OA). METHODS: OA models were established. Normal model, artesunate, and Viatril-S groups (20 rats respectively) were set. Enzyme-linked immunosorbent assay, IHC staining, and quantitative real-time polymerase chain reaction were conducted to calculate IGF-1, OPN, and CTX-II levels in serum, SF, and cartilage tissues of rats. The pathological changes in cartilage tissues were evaluated with Mankin score and Hematoxylin-Eosin staining. RESULTS: Compared with the normal group, the model group showed increased IGF-1 level; decreased OPN, CTX-II levels in the serum and SF; and contrary results were seen in the cartilage tissues. A gradual ascending IGF-1 level and descending OPN and CTX-II levels existed in the serum and SF in the artesunate and Viatril-S groups after 2 weeks. The model group showed the most obvious pathological changes and highest Mankin score compared with the other groups. Higher IGF-1 level and lower OPN, CTX-II levels were exhibited in the cartilage tissue in the artesunate and Viatril-S groups but not in the model group. CONCLUSION: Artesunate and Viatril-S inhibit OA development by elevating IGF-1 level and reducing OPN and CTX-II levels. (C) 2017 S. Karger AG, Basel