The fatty acid synthase (FAS) gene and its promoter in Rattus norvegicus.
The fatty acid synthase (FAS) gene and its promoter in Rattus norvegicus.
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褐家鼠脂肪酸合酶（FAS）基因及其启动子。
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
M. Schweizer
中科院分区：
文献类型：
--
作者：
K. Beck;R. Schreglmann;I. Stathopulos;H. Klein;J. Hoch;M. Schweizer
对大鼠肝脏基因组文库进行筛选，得到5个大鼠脂肪酸合成酶(Fas)基因的重叠克隆。从这些克隆中，我们测定了大鼠Fas的18,170个碱基的序列，以及5‘-侧翼区的5,028个碱基和3’-邻接的基因组序列的515个碱基。这两个Fas转录本由一个未翻译外显子和42个已翻译外显子组成，仅在其多聚腺化/终止位点的位置上有所不同。令人惊讶的是，Enoyl还原酶的底物结合位点被一个内含子打断。各个结构域的大小和边界可以与基因的外显子/内含子结构相关联。这八个部分函数与外显子的离散单位重合。酰基载体蛋白及其假体4‘-磷酸丙氨酸基团位于单个外显子内，支持大鼠Fas是通过基因融合进化的这一观点。经扩增后，Fas基因在肝和乳腺组织中的主要转录起始点位于5,028个碱基。正如预期的那样，受翻译前调控的基因的5‘侧翼区域除了TATA和CAAT盒外，还包含几个DNA结合蛋白的共同序列。
Screening of rat liver genomic libraries yielded 5 overlapping clones for rat fatty acid synthase (FAS). From these clones we determined the 18,170 bp sequence of the rat FAS together with 5,028 bp of the 5'-flanking region and 515 bp of the 3'-adjacent genomic sequence. The two FAS transcripts which differ only in the positions of their polyadenylation/termination sites consist of one untranslated and 42 translated exons. Surprisingly, the substrate binding site for enoyl reductase, one of the FAS component functions, is interrupted by an intron. The sizes and the boundaries of the individual domains could be mapped in relation to the exon/intron structure of the gene. These eight partial functions coincide with discrete units of exons. The acyl carrier protein with its prosthetic 4'-phosphopantetheine group is located within a single exon supporting the idea that rat FAS has evolved by gene fusion. Using primer extension the main transcription start site of the FAS mRNA in both hepatic and mammary gland tissues was located at 5,028 bp in the sequence determined. As expected of a gene which is pretranslationally regulated the 5'-flanking region contains, in addition to TATA and CAAT boxes, consensus sequences for several DNA binding proteins.
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DOI：
10.1111/j.1432-1033.1991.tb16052.x
发表时间：
1991-06
期刊：
European journal of biochemistry
影响因子：
--
作者：
A. Witkowski;V. Rangan;Z. Randhawa;C. Amy;S. Smith
通讯作者：
A. Witkowski;V. Rangan;Z. Randhawa;C. Amy;S. Smith
DOI：
10.1073/pnas.84.14.4767
发表时间：
1987-07-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
DOI：
10.1016/s0021-9258(18)88885-8
发表时间：
1985-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
W. K. Jones;L. Yu-Lee;S. Clift;T. L. Brown;J. Rosen
通讯作者：
W. K. Jones;L. Yu-Lee;S. Clift;T. L. Brown;J. Rosen
影响因子：
56.9
作者：
BRIGGS, MR;KADONAGA, JT;TJIAN, R
通讯作者：
TJIAN, R
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kameda,K;Goodridge,AG
通讯作者：
Goodridge,AG