Non-coenzyme role of vitamin B1 in RANKL-induced osteoclastogenesis and ovariectomy induced osteoporosis
Non-coenzyme role of vitamin B1 in RANKL-induced osteoclastogenesis and ovariectomy induced osteoporosis
复制标题
维生素 B1 在 RANKL 诱导的破骨细胞生成和卵巢切除诱导的骨质疏松中的非辅酶作用
DOI：
10.1002/jcb.29632
复制
发表时间：
2020
影响因子：
4
通讯作者：
Dou Ce
中科院分区：
文献类型：
--
作者：
Ma Qinyu;Liang Mengmeng;Wang Yaxi;Ding Ning;Wu Yutong;Duan Lianli;Yu Tao;Lu Yanzhu;Xu Jianzhong;Kang Fei;Dou Ce
Vitamins B are co‐enzymes participating in energy metabolic pathways. While some vitamins B are known affecting bone homeostasis, the effects of vitamin B1 (thiamine) on bone health remains unclear. In our study, we used cell counting kit‐8, tartrate‐resistant acid phosphatase stain, actin cytoskeleton stain, and pit formation assay to evaluate the effect of thiamine on osteoclast differentiation, formation, and function, respectively. Then we used dichloro‐dihydro‐fluorescein diacetate assay to investigate reactive oxygen species (ROS) generation and removal. Osteoporosis model by ovariectomy was established for animal experiments. We found that thiamine had inhibitory effect on osteoclast differentiation. And its inhibitory role on osteoclast differentiation is in a dose‐dependent way. Mechanistically, ThDP suppresses intracellular ROS accumulation and unfolded protein response signaling during osteoclastogenesis via inhibiting Rac‐Nox1/2/4 and intracellular inositol‐requiring protein‐1α/X‐box‐binding protein pathways, respectively. Osteoporotic mice treated with thiamine rich dietary showed better bone strength relative to thiamine deficient dietary. Our study explored the non‐coenzyme inhibitory functions of B1 vitamin in receptor activator of nuclear factor κB ligand induced osteoclastogenesis and uncovered the significance of B1 vitamin in bone health.
