Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of phosphorylated compounds using a novel phosphate capture molecule
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of phosphorylated compounds using a novel phosphate capture molecule
复制标题
DOI：
10.1002/rcm.1154
复制
发表时间：
2003-01-01
影响因子：
2
通讯作者：
Koike, T
中科院分区：
文献类型：
--
作者：
Takeda, H;Kawasaki, A;Koike, T
本文介绍了一种简单、快速、灵敏的基质辅助激光解吸电离飞行时间质谱（MALDI-TOFMS）分析磷酸化化合物（ROPO 32-）的方法。该方法是基于一个特征的质量转移和总电荷的变化由于ROPO 32-与双核锌（II）络合物的络合，磷酸盐残基的浓度从-2到+1（1，3-双[双（2-吡啶基甲基）氨基]-2-丙醇合二锌（II）络合物，Zn 2L 3+）。此外，使用单一的锌同位素衍生物（（Zn 2L 3+）-Zn-64和（ZnL 3+）-Zn-68-L-2）能够提高分析的灵敏度和准确度。版权所有（C）2003约翰威利父子有限公司。
This paper introduces a simple, rapid, and sensitive procedure for the analysis of phosphorylated compounds (ROPO32-) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The method is based on a characteristic mass shift and a total-charge change (from -2 to +1) of the phosphate residue due to complexation of ROPO32- with a dinuclear zinc(II) complex (1,3-bis[bis(2-pyridinylmethyl)amino]-2-propanolato dizinc(II) complex, Zn2L3+) in aqueous solution at physiological pH. Furthermore, the use of single zinc-isotope derivatives ((Zn2L3+)-Zn-64 and (ZnL3+)-Zn-68-L-2) enables improvement of the sensitivity and accuracy of the analysis. Copyright (C) 2003 John Wiley Sons, Ltd.