HETEROTOPIC BONE FORMATION BY NANO-APATITE CONTAINING POLY(D,L-LACTIDE) COMPOSITES
HETEROTOPIC BONE FORMATION BY NANO-APATITE CONTAINING POLY(D,L-LACTIDE) COMPOSITES
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DOI：
10.22203/ecm.v019a24
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Yuan, H.
中科院分区：
文献类型：
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作者：
Barbieri, D.;Renard, A. J. S.;Yuan, H.
为了使聚合物材料具有骨诱导性，将纳米级磷酸钙磷灰石颗粒（CaP）引入低分子量聚乳酸（D， l -丙交酯）。分别以重量比为10%、20%和40%的磷灰石含量制备均相复合材料，以纯聚乳酸为对照。然后制备多孔样品（孔径300-400 μ m，孔隙率60%）并灭菌。体外研究表明，钙离子从复合材料中释放出来取决于磷灰石含量，而在模拟体液（SBF）中，14天内仅在40% CaP复合材料上观察到表面矿物沉积。在犬肌内植入12周后，只有40% CaP复合植入物保持其形状，并在孔隙内出现异位骨形成。综上所述，在聚（D， l -丙交酯）中添加40%的磷灰石可获得骨诱导材料。未来的研究将集中在了解这种材料定向骨诱导现象，以开发一种有前途的骨移植替代品。
To render polymeric materials osteoinductive, nano-sized calcium phosphate apatite particles (CaP) were introduced into a low molecular weight poly(D, L-lactide). Homogenous composites were made with 10%, 20% and 40% by weight of apatite content while pure polylactide was used as control. Thereafter porous samples (pore size 300-400 mu m, 60% porosity) were fabricated and sterilized. In vitro studies showed that calcium ions were released from the composites depending on the apatite content, while surface mineral deposition was observed only on the 40% CaP composites in simulated body fluid (SBF) within 14 days. After 12 weeks of intramuscular implantation in dogs, only the 40% CaP composite implant retained its shape and showed ectopic bone formation within the pores. In conclusion, adding a content of 40% apatite into poly(D,L-lactide) could lead to an osteoinductive material. Future studies will focus on understanding this phenomenon of material-directed osteoinduction in order to develop a promising bone graft substitute.