Assessment of Lymph Node Stromal Cells as an Underlying Factor in Age-Related Immune Impairment
Assessment of Lymph Node Stromal Cells as an Underlying Factor in Age-Related Immune Impairment
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DOI：
10.1093/gerona/glz029
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发表时间：
2019-11-01
影响因子：
5.1
通讯作者：
Haynes, Laura
中科院分区：
文献类型：
--
作者：
Masters, April R.;Hall, Alexxus;Haynes, Laura
衰老对免疫力产生负面影响，导致对疫苗接种和感染的反应效率低下。成纤维细胞网状细胞（FRC）是淋巴结（LN）中主要的基质细胞亚群，在协调和控制适应性免疫应答中起着复杂的作用。虽然基质细胞对免疫应答有重要影响，但衰老对LN基质细胞的影响仍不清楚。通过流式细胞术定量分析LN基质细胞显示，在稳定状态下年轻和老年LN中基质细胞的数量没有显著差异，但在流感感染后，老年FRC由于增殖减少而延迟扩增。老化的LN还产生降低水平的稳态趋化因子，这与幼稚T细胞归巢减少相关。图像分析显示，年轻和老年T细胞区FRC在稳态和感染后具有相似的形态。此外，老年FRC似乎不是流感感染后转移到老年LN的年轻T细胞增殖减少的一个促成因素。这些结果表明，老化改变LN基质细胞对挑战的反应，这些年龄相关的变化可能是老年人免疫反应受损的潜在因素。
Aging negatively impacts immunity, resulting in inefficient responses to vaccinations and infections. Fibroblastic reticular cells (FRCs) are the major stromal cell subset in lymph nodes (LNs) and play an intricate role in the orchestration and control of adaptive immune responses. Although stromal cells have a major impact on immune responses, the impact of aging on LN stromal cells remains unclear. Quantitative analysis of LN stromal cells by flow cytometry revealed that there are no significant differences in the number of stromal cells in young and aged LN at steady state but after influenza infection aged FRCs have delayed expansion as a result of reduced proliferation. Aged LNs also produce reduced levels of homeostatic chemokines, which correlates with reduced homing of naive T cells. Image analysis reveals that young and aged T-cell zone FRCs have similar morphology at steady state and after infection. Furthermore, aged FRCs did not appear to be a contributing factor in the reduced proliferation of young T cells transferred into aged LNs after influenza infection. These results demonstrate that aging alters LN stromal cell response to challenge and these age-related changes may be an underlying contributor to impaired immune responses in the elderly people.