A Synthetic Approach Reveals Extensive Tunability of Auxin Signaling
A Synthetic Approach Reveals Extensive Tunability of Auxin Signaling
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DOI：
10.1104/pp.112.202184
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发表时间：
2012-09-01
期刊：
影响因子：
7.4
通讯作者：
Nemhauser, Jennifer L.
中科院分区：
文献类型：
--
作者：
Havens, Kyle A.;Guseman, Jessica M.;Nemhauser, Jennifer L.
解释小分子生长素如何触发多样化但特定的反应是植物生物学中长期存在的挑战。生长素反应的一个重要步骤是通过与生长素受体相互作用来降解生长素/吲哚-3-乙酸（Aux/IAA，以下简称IAA）阻遏蛋白。为了系统地表征IAA垂直杆受体对之间降解行为的多样性，我们在酵母（酿酒酵母）中设计了生长素诱导的植物IAA蛋白降解。我们发现 IAA 降解动力学差异很大，具体取决于存在的受体，并且 IAA 不仅仅由包含降解决定子的结构域 II 编码。为了促进这项研究和未来的研究，我们确定了一个数学模型，能够在单个参数中定量描述 IAA 降解行为。总之，我们的结果证明了生长素响应途径的特定配置所赋予的显着可调性。
Explaining how the small molecule auxin triggers diverse yet specific responses is a long-standing challenge in plant biology. An essential step in auxin response is the degradation of Auxin/Indole-3-Acetic Acid (Aux/IAA, referred to hereafter as IAA) repressor proteins through interaction with auxin receptors. To systematically characterize diversity in degradation behaviors among IAA vertical bar receptor pairs, we engineered auxin-induced degradation of plant IAA proteins in yeast (Saccharomyces cerevisiae). We found that IAA degradation dynamics vary widely, depending on which receptor is present, and are not encoded solely by the degron-containing domain II. To facilitate this and future studies, we identified a mathematical model able to quantitatively describe IAA degradation behavior in a single parameter. Together, our results demonstrate the remarkable tunability conferred by specific configurations of the auxin response pathway.