A study of myc-related gene expression in small cell lung cancer by in situ hybridization.
A study of myc-related gene expression in small cell lung cancer by in situ hybridization.
复制标题
原位杂交研究小细胞肺癌myc相关基因表达。
DOI：
--
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
I. Kirsch
中科院分区：
文献类型：
--
作者：
J. Gu;R. Linnoila;N. L. Seibel;A. Gazdar;J. Minna;B. Brooks;G. Hollis;I. Kirsch
应用RNA-RNA组织原位杂交技术研究了小细胞肺癌（SCLC）中myc相关基因（c-myc、N-myc和L-myc）的表达。研究的组织包括来自SCLC患者的10个细胞系的细胞离心涂片、来自细胞系的4个相应裸鼠异种移植物以及通过手术活检和尸检获得的转移性肿瘤组织。将探针制备为35 S标记的互补RNA。通过放射自显影术在肿瘤细胞样品的细胞质中特异性地证明每个基因的表达。每个样本中癌基因表达的平均水平证实了先前通过北方印迹分析获得的数据。此外，注意到每个样品中细胞间基因表达的异质性。本研究首次尝试在肺癌细胞系和组织中原位表达癌基因，并证实这些myc相关基因的表达可以在原发肿瘤中看到。RNA-RNA组织原位杂交技术在回答小细胞肺癌肿瘤细胞异质性和进展的基本问题方面具有巨大潜力。它在前瞻性和回顾性研究中应该是有用的。
The expression of myc-related genes (c-myc, N-myc, and L-myc) in small cell lung cancer (SCLC) was studied by RNA-RNA tissue in situ hybridization. The tissues investigated included cytospins of ten cell lines derived from patients with SCLC, four corresponding nude mouse xenografts from cell lines, and metastatic tumor tissue obtained by surgical biopsy and at autopsy. The probes were prepared as 35S labeled complementary RNA. The expression of each gene was demonstrated specifically by autoradiography in the cytoplasm of the neoplastic cell samples. The average levels of oncogene expression in each specimen corroborated previous data obtained by Northern blot assays. In addition, heterogeneity in gene expression from cell to cell in each sample was noted. This study represents the first attempt to demonstrate oncogene expression in lung cancer cell lines and tissues in situ, and confirms that the expression of these myc related genes can be seen in the primary tumor. The technique of RNA-RNA tissue in situ hybridization has great potential in answering fundamental questions of tumor cell heterogeneity and progression in SCLC. It should be useful in both prospective and retrospective studies.