A novel cell adhesion molecule, G-CAM, found on cultured rat glia.
A novel cell adhesion molecule, G-CAM, found on cultured rat glia.
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在培养的大鼠神经胶质细胞上发现了一种新型细胞粘附分子 G-CAM。
DOI：
10.1016/0304-3940(91)90584-g
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发表时间：
1991
影响因子：
2.5
通讯作者：
Larjava,H
中科院分区：
文献类型：
--
作者：
GeisertJr,EE;Murphy,TP;Irwin,MH;Larjava,H
使用单克隆抗体（mAb），指定AMP 1，一种新的细胞粘附分子被确定为大鼠星形胶质细胞和少突胶质细胞。当培养的大鼠星形胶质细胞的汇合单层被标记与AMP 1，抗原被离散地定位于细胞表面的区域中的细胞-细胞接触。该抗体没有标记接种在新生星形胶质细胞单层上的胚胎大鼠皮质神经元，表明该抗原既不存在于培养的神经元上，也不重组神经元下的神经胶质表面。在星形胶质细胞或脑蛋白的免疫印迹上，mAb AMP 1识别106 kDa的蛋白。在本文中，数据表明，AMP 1抗原是一种细胞粘附分子，并不同于所有已知的细胞粘附分子存在于星形胶质细胞：N-CAM，N-cadherin，或β 1整合素家族的成员。我们暂时将这种分子命名为“胶质细胞粘附分子”（G-CAM）。
Using a monoclonal antibody (mAb), designated AMP1, a novel cell adhesion molecule was identified on rat astrocytes and oligodendrocytes. When confluent monolayers of cultured rat astrocytes were labeled with AMP1, the antigen was discretely localized to the cell surface in regions of cell-cell contacts. The antibody did not label embryonic rat cortical neurons plated on monolayers of neonatal astrocytes, indicating that the antigen is neither present on cultured neurons nor does it reorganize on the glial surface under the neurons. On immunoblots of astrocytic or brain proteins, mAb AMP1 recognized a 106 kDa protein. In the present paper, data are presented demonstrating that the AMP1 antigen is a cell adhesion molecule and is distinct from all the known cell adhesion molecules present on astrocytes: N-CAM, N-cadherin, or members of theβ1intergrin family. We have tentatively termed this molecule ‘glial cell adhesion molecule’ (G-CAM).