Adventitial myofibroblasts play no major role in neointima formation after angioplasty
Adventitial myofibroblasts play no major role in neointima formation after angioplasty
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DOI：
10.1080/14017430310007018
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发表时间：
2003-01-01
影响因子：
2.2
通讯作者：
Nielsen, S
中科院分区：
文献类型：
--
作者：
Maeng, M;Mertz, H;Nielsen, S
目的 - 血管成形术后，从外膜迁移的肌成纤维细胞被认为构成了新生内膜细胞的大部分。我们试图通过使用结蛋白（smoothelin）来检验这一假设，结蛋白是静止平滑肌细胞（SMC）表型的标志物，而肌成纤维细胞不表达该标志物。
设计 - 对25只兔子的左髂动脉进行球囊血管成形术，这些兔子在3 - 56天后处死。对动脉横断面进行α - 肌动蛋白（通用标志物）、结蛋白（静止SMC表型）和Ki - 67（增殖表型）免疫染色。
结果 - 外膜细胞短暂地呈现肌动蛋白阳性（肌成纤维细胞），但在任何时间点都不表达结蛋白。在中膜，血管成形术诱导了短暂的增殖，同时结蛋白表达短暂下降。血管成形术后7天出现的新生内膜细胞最初处于增殖状态且结蛋白阴性，但在56天后转变为非增殖、结蛋白阳性细胞，此时82±10%的细胞结蛋白染色阳性。中膜和内膜细胞的这种表型调节始于中膜，并逐渐向管腔移动。
结论 - 在后期随访中，大多数内膜细胞结蛋白阳性，这表明外膜肌成纤维细胞对新生内膜的形成没有重要作用。
Objective-Myofibroblasts migrating from adventitia have been suggested to constitute a majority of neointimal cells after angioplasty. We sought to examine this hypothesis by use of smoothelin, which is a marker for the quiescent smooth muscle cell (SMC) phenotype while not expressed by myofibroblasts.Design-Balloon angioplasty was performed in left iliac arteries of 25 rabbits that were killed after 3-56 days. Arterial cross-sections were immunostained for alpha-actin (general marker), smoothelin (quiescent SMC phenotype), and Ki-67 (proliferative phenotype).Results-Adventitial cells became transiently actinpositive (myofibroblasts) but did not express smoothelin at any time point. In media, angioplasty induced transient proliferation and coinciding transient decrease in smoothelin expression. Neointimal cells, present 7 days after angioplasty, were initially proliferating and smoothelin-negative but changed to non-proliferating, smoothelin-positive cells after 56 days where 82+/-10% of cells stained positive for smoothelin. This phenotypic modulation of medial and intimal cells began in media and moved gradually towards the lumen.Conclusion-At late follow-up, the majority of intimal cells are smoothelin-positive indicating that adventitial myofibroblasts play no major role for neointima formation.